Linked Life Data

7607539

Source:http://linkedlifedata.com/resource/pubmed/id/7607539

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-8-15
pubmed:databankReference
pubmed:abstractText
A complete cDNA sequence (1026 bp) and a partial structure of a gene encoding the trout testis chromosomal HMG (high mobility group) 2 protein (HMG-T2) is presented. The deduced protein consists of 214 amino acids and shares over 80% similarity to the trout HMG-1 protein (HMG-T1) as well as to the mammalian or avian HMG-2 proteins. Northern blot analysis revealed two transcripts, a major one of 1.2 kb and a minor one of 1.6 kb. Southern analysis and polymerase chain reaction of trout genomic DNA indicated that the HMG-T2 gene is encoded by several introncontaining genes. The 5'-UTR (untranslated region) of the HMG-T2 is interrupted by an intron and the coding region of the HMG-T2 is divided into four exons by three relatively short introns (173, 91 and 78 bp). The exon/intron boundaries of trout HMG-2 are identical to those of human HMG-2, as reported earlier [Shirakawa and Yoshida, J. Biol. Chem. 267 (1992) 6641-6645], suggesting the evolution of the HMG-1/2 family genes from a common ancestor. Phylogenetic analysis indicated that the common ancestor of trout HMG-1/2 genes very likely diverged from the ancestor of the mammalian (or avian) HMG-1/2 genes before its separation into two distinct mammalian or (avian) HMG-1 and HMG-2 genes. Sequence comparisons of the 3'UTR of trout HMG-2 cDNA with the corresponding regions in the mammalian (or avian) HMG-2 revealed that the trout 3'-UTR was clearly distinct from the 3'-UTR of the mammalian or avian HMG-2 cDNAs which were otherwise remarkably well conserved.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
158
pubmed:geneSymbol
HMG-2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
181-7
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:7607539-Amino Acid Sequence, pubmed-meshheading:7607539-Animals, pubmed-meshheading:7607539-Base Sequence, pubmed-meshheading:7607539-Biological Evolution, pubmed-meshheading:7607539-Blotting, Northern, pubmed-meshheading:7607539-Blotting, Southern, pubmed-meshheading:7607539-DNA, Complementary, pubmed-meshheading:7607539-Exons, pubmed-meshheading:7607539-Genome, pubmed-meshheading:7607539-High Mobility Group Proteins, pubmed-meshheading:7607539-Introns, pubmed-meshheading:7607539-Male, pubmed-meshheading:7607539-Molecular Sequence Data, pubmed-meshheading:7607539-Polymerase Chain Reaction, pubmed-meshheading:7607539-Protein Biosynthesis, pubmed-meshheading:7607539-Sequence Homology, Amino Acid, pubmed-meshheading:7607539-Species Specificity, pubmed-meshheading:7607539-Testis, pubmed-meshheading:7607539-Trout
pubmed:year
1995
pubmed:articleTitle
cDNA sequence and structure of a trout HMG-2 gene. Evidence for a trout-specific 3'-untranslated region.
pubmed:affiliation
Department of Biochemistry, University of Calgary, Alberta, Canada.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't