Linked Life Data

760361

Source:http://linkedlifedata.com/resource/pubmed/id/760361

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1979-3-13
pubmed:abstractText
Antisera were raised to 1-34 hPTH-B and to 1-34 hPTH-N. In the assays utilizing antisera to 1-34 hPTH-B the relative affinity of 1-84 hPTH was correlated with the activity displayed by 1-34 hPTH-N. The activity of 13-34 hPTH-B was always higher than that of 1-12 hPTH, but both peptides displaced most of the radioiodinated 1-34 hPTH-B from the antiserum. This may be due to the fact that the 1-12 and 13-34 fragments contain a homologous sequence: 6--10 (-GlN-Leu-Met-His-AsN-) and 29--33 (-GlN-Leu-Val-His-AsN-). It was possible to develop a heterologous radioimmunoassay using anti-(1-34 hPTH-B) antiserum and [125I]1-34 hPTH-N with favourable properties: complete cross-reactivity of 1-84 hPTH and high sensitivity (less than 10-14 mol/tube). All antisera to 1-34 hPTH-N were characterized by a high reactivity towards 1-34 bPTH and a higher avidity for 1-84 hPTH than for 1-34 hPTH-B. In all assays using either antisera to 1-34 hPTH-B or antisera to 1-34 hPTH-N 19-24 hPTH-B was completely inactive. In accordance with our previous observation that position 30 in native hPTH is probably occupied by an aspartic acid residue, the present study seems to indicate that position 28 or 30 is indeed represented incorrectly by the structure of 1-34 hPTH-B.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0001-5598
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
90-102
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Production and characterization of antisera to synthetic 1-34 human parathyroid hormone fragments: possible implications for the correctness of proposed structures.
pubmed:publicationType
Journal Article