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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-8-10
pubmed:abstractText
Antimicrobial resistance patterns (ARP), virulence profiles and plasmids in clinical isolates of Yersinia enterocolitica serotype O:3 were studied. The ARP was tested using the disk method. All strains were susceptible to amoxicillin/clavulanic acid, cefoxitin, fosfomycin, gentamicin, kanamycin, neomycin, tetracycline, nalidixic acid, norfloxacin, ciprofloxacin and trimethoprim. All of them presented resistance to ampicillin, all with the exception of one to cephalotin, differing in resistance or susceptibility to chloramphenicol, streptomycin (Sm), sulfadiazine (Sd) and cotrimoxazole. Due to these differences they were grouped into 8 ARP. Twenty-nine strains carried plasmids and were grouped into 5 plasmid profiles. All strains carrying 42 MDa plasmids were positive for virulence tests (calcium dependence, crystal violet binding, Congo red binding and autoagglutination). No correlation between ARP and plasmid profile was found, although small plasmids of 6.5 and 4.1 MDa mediated resistance to Sm-Sd, as was shown by transformation to Escherichia coli strains. DNAs from plasmids were analyzed by restriction enzymes. All 42 MDa plasmids showed identical EcoRI and HindIII profiles. The two 6.5 MDa plasmids showed identical BglII, AvaI and SalI restriction profiles and the four 4.1 Mda plasmids also yielded restriction profiles similar to each other, but different from the 6.5 ones.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1121-7138
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
215-22
pubmed:dateRevised
2011-2-25
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Yersinia enterocolitica O:3. Antimicrobial resistance patterns, virulence profiles and plasmids.
pubmed:affiliation
Departamento de Biología Funcional, Area Microbiología, Universidad de Oviedo, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't