| pubmed-article:7601146 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7601146 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:7601146 | lifeskim:mentions | umls-concept:C0021585 | lld:lifeskim |
| pubmed-article:7601146 | lifeskim:mentions | umls-concept:C0027934 | lld:lifeskim |
| pubmed-article:7601146 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:7601146 | lifeskim:mentions | umls-concept:C0005678 | lld:lifeskim |
| pubmed-article:7601146 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:7601146 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7601146 | pubmed:dateCreated | 1995-8-10 | lld:pubmed |
| pubmed-article:7601146 | pubmed:abstractText | alpha-latrotoxin, alpha-latroinsectotoxin and the low-molecular-mass protein from black widow spider venom were synthesised in insect cells using the baculovirus expression system. SDS/PAGE analysis of recombinant-virus-infected cells revealed novel proteins that migrated with sizes similar to those of the neurotoxins from spider venom. The identities of these proteins as alpha-latrotoxin, alpha-latroinsectotoxin or the low-molecular-mass protein were confirmed by immunoblot analysis of infected cells with anti-(alpha-latrotoxin), anti-(alpha-latroinsectotoxin) or anti-(low-molecular-mass protein) IgG. Neither the low-molecular-mass protein nor alpha-latrotoxin were toxic upon injection into Trichoplusia ni larvae or upon virus-derived synthesis directly in the cytoplasm of the target tissue. Analysis of the biological activity of the recombinant virus encoding alpha-latroinsectotoxin, however, revealed a strong toxic effect on the T. ni larvae. These data indicate that the toxic effect of the native insectotoxin may be promoted by the alpha-latroinsectotoxin subunit alone and provides evidence that the mechanism of action of alpha-latroinsectotoxin may be mediated by internalisation of part of the neurotoxin alpha-subunit molecule. | lld:pubmed |
| pubmed-article:7601146 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7601146 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7601146 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7601146 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7601146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7601146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7601146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7601146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7601146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7601146 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7601146 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:7601146 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:7601146 | pubmed:author | pubmed-author:BeadleD JDJ | lld:pubmed |
| pubmed-article:7601146 | pubmed:author | pubmed-author:KingL ALA | lld:pubmed |
| pubmed-article:7601146 | pubmed:author | pubmed-author:GrishinE VEV | lld:pubmed |
| pubmed-article:7601146 | pubmed:author | pubmed-author:KiyatkinN INI | lld:pubmed |
| pubmed-article:7601146 | pubmed:author | pubmed-author:KulikovskayaI... | lld:pubmed |
| pubmed-article:7601146 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7601146 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:7601146 | pubmed:volume | 230 | lld:pubmed |
| pubmed-article:7601146 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7601146 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7601146 | pubmed:pagination | 854-9 | lld:pubmed |
| pubmed-article:7601146 | pubmed:dateRevised | 2009-9-29 | lld:pubmed |
| pubmed-article:7601146 | pubmed:meshHeading | pubmed-meshheading:7601146-... | lld:pubmed |
| pubmed-article:7601146 | pubmed:meshHeading | pubmed-meshheading:7601146-... | lld:pubmed |
| pubmed-article:7601146 | pubmed:meshHeading | pubmed-meshheading:7601146-... | lld:pubmed |
| pubmed-article:7601146 | pubmed:meshHeading | pubmed-meshheading:7601146-... | lld:pubmed |
| pubmed-article:7601146 | pubmed:meshHeading | pubmed-meshheading:7601146-... | lld:pubmed |
| pubmed-article:7601146 | pubmed:meshHeading | pubmed-meshheading:7601146-... | lld:pubmed |
| pubmed-article:7601146 | pubmed:meshHeading | pubmed-meshheading:7601146-... | lld:pubmed |
| pubmed-article:7601146 | pubmed:meshHeading | pubmed-meshheading:7601146-... | lld:pubmed |
| pubmed-article:7601146 | pubmed:meshHeading | pubmed-meshheading:7601146-... | lld:pubmed |
| pubmed-article:7601146 | pubmed:meshHeading | pubmed-meshheading:7601146-... | lld:pubmed |
| pubmed-article:7601146 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7601146 | pubmed:articleTitle | Functional characterization of black widow spider neurotoxins synthesised in insect cells. | lld:pubmed |
| pubmed-article:7601146 | pubmed:affiliation | School of Biological and Molecular Sciences, Oxford Brookes University, England. | lld:pubmed |
| pubmed-article:7601146 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7601146 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7601146 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7601146 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7601146 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7601146 | lld:pubmed |
