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rdf:type |
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lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0019630,
umls-concept:C0035820,
umls-concept:C0039194,
umls-concept:C0205251,
umls-concept:C0332120,
umls-concept:C0596448,
umls-concept:C0871261,
umls-concept:C1510827,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
8
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pubmed:dateCreated |
1995-8-10
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pubmed:abstractText |
The crystallographic structure of the MHC class II molecule showed that the alpha beta heterodimer can itself dimerize to form a four chain (alpha beta)2 complex of 120 kDa. Here we provide evidence for the existence of a 120 kDa (alpha beta)2 complex of the class II I-Ek molecules in mouse B cells. Both a 60 kDa and a 120 kDa form of I-Ek are detected by Western blotting and by immunoprecipitation under conditions in which class II alpha beta heterodimers are stable. The 120 kDa I-Ek complex does not contain Ii and, upon warming, dissociates into free alpha and beta chains. The 120 kDa I-Ek complex is expressed at the cell surface, is active in antigen presentation, and appears to play a significant role in T cell responses to low affinity but not to high affinity antigens, possibly by facilitating cross-linking of the T cell receptors.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1074-7613
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
699-707
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7600295-Animals,
pubmed-meshheading:7600295-Antibodies, Monoclonal,
pubmed-meshheading:7600295-Antigen Presentation,
pubmed-meshheading:7600295-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:7600295-B-Lymphocytes,
pubmed-meshheading:7600295-Blotting, Western,
pubmed-meshheading:7600295-Cell Line,
pubmed-meshheading:7600295-Cell Membrane,
pubmed-meshheading:7600295-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7600295-Flow Cytometry,
pubmed-meshheading:7600295-Histocompatibility Antigens Class II,
pubmed-meshheading:7600295-Lymphocyte Activation,
pubmed-meshheading:7600295-Mice,
pubmed-meshheading:7600295-Precipitin Tests,
pubmed-meshheading:7600295-Protein Conformation,
pubmed-meshheading:7600295-Spleen,
pubmed-meshheading:7600295-T-Lymphocytes,
pubmed-meshheading:7600295-Time Factors
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pubmed:year |
1994
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pubmed:articleTitle |
Evidence for dimers of MHC class II molecules in B lymphocytes and their role in low affinity T cell responses.
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pubmed:affiliation |
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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