Linked Life Data

7597698

Source:http://linkedlifedata.com/resource/pubmed/id/7597698

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-8-3
pubmed:abstractText
Micromass culture systems of mesencephalon (CNS) and limb bud (LB) regions were used to evaluate the impact of the O6-methylguanine-DNA methyltransferase (MGMT) inhibitor, O6-benzylguanine (BG), on ethylnitrosourea (ENU) and methylnitrosourea (MNU)-induced in vitro toxicity. These primary rat embryo cells were preincubated with BG (2.5 microM for CNS; 10 microM for LB) for 24 hr and then ENU (0-1708 microM) or MNU (0-1600 microM) was added. Five days after culture initiation, cell survival was assessed using neutral red uptake and cell differentiation was assessed using selective staining (Alcian blue in LB cultures and hematoxylin in CNS) and image analysis. In both LB and CNS cultures, preexposure to BG resulted in a significant increase in inhibition of differentiation following MNU exposure but no increase in cytotoxicity. There were no statistically significant changes in the toxicity induced by ENU in these differentiating cells consequent to BG preexposure. Our observations lend support to the idea of a specific role for DNA adducts in defining MNU's toxic effects on cell viability and differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:volume
133
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-11
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Modulation of nitrosourea toxicity in rodent embryonic cells by O6-benzylguanine, a depletor of O6-methylguanine-DNA methyltransferase.
pubmed:affiliation
Armstrong Laboratory, Wright-Patterson Air Force Base, Dayton, Ohio 45433, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.