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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1995-8-3
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pubmed:abstractText |
Site-specific recombination systems are useful tools for chromosome engineering in vivo and site-specific DNA cleavage methods have applications in genome analysis and gene isolation. Here, we report a new method to fragment chromosomes in vitro using the Cre-lox site-specific recombination system. Two lox sites were targeted into the 5.7 Mb chromosomes I of Schizosaccharomyces pombe. In vitro recombination between chromosomal lox sites and exogenously provided lox oligonucleotides 'cleaved' the chromosome at the defined lox sequences. Site-specific cleavage of lox sites in the tobacco genome was also demonstrated. This recombination-based cleavage method provides a novel approach for structural and functional analyses of eukaryotic chromosomes as it allows direct isolation of chromosome regions that correspond to phenotypes revealed through Cre-lox mediated chromosome rearrangements in vivo. Moreover, recombination with end-labeled lox oligonucleotides would permit the specific end-labeling of chromosome segments to facilitate the long range mapping of chromosomes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-1292893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-1310059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-1317550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-1448066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-1454542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-1628809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-1836279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-1848684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-1962209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-2035025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-2218529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-2404945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-2479980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-2555261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-2660260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-3071260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-6319400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-7885845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-8052855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-8122308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-8127869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-8219087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-8392474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-8404527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-8438159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-8462094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7596819-8502555
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1923-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1995
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pubmed:articleTitle |
Site-specific cleavage of chromosomes in vitro through Cre-lox recombination.
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pubmed:affiliation |
Plant Gene Expression Center, US Department of Agriculture, Albany, CA 94710, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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