Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-12-28
pubmed:abstractText
We studied the influence of conjugation methods and storage conditions on the stability of immunoconjugates with peroxidase. We demonstrate here that conjugates formed by the maleimide-sulfhydryl method and by the periodate oxidation method lose activity when maintained in diluted solutions. However, while the loss of activity of MSM conjugates is due exclusively to hydrolysis of the thioether bond, the loss of activity of periodate complexes is caused by a reduction of both the enzymatic and antibody immunochemical activities. Based on these observations, we developed a buffer that stabilizes the thioether bond, thus permitting long time storage of these immunoconjugates at low concentration and at above freezing temperatures.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0197-1522
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-24
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Influence of the antibody-peroxidase coupling methods on the conjugate stability and on the methodologies for the preservation of the activity in time.
pubmed:affiliation
Sclavo Diagnostics Srl, R/D Department, Siena, Italy.
pubmed:publicationType
Journal Article