Linked Life Data

7592809

Source:http://linkedlifedata.com/resource/pubmed/id/7592809

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
44
pubmed:dateCreated
1995-12-21
pubmed:databankReference
pubmed:abstractText
Insulin secretion is associated with changes in pancreatic beta-cell K+ permeability. A degenerate polymerase chain reaction strategy based on the conserved features of known inwardly rectifying K+ (KIR) channel genes was used to identify members of this family expressed in human pancreatic islets and insulinoma. Three related human KIR transcript sequences were found: CIR (also known as cardiac KATP-1), GIRK1, and GIRK2 (KATP-2). The pancreatic islet CIR and GIRK2 full-length cDNAs were cloned, and their genes were localized to human chromosomes 11q23-ter and 21, respectively. Northern blot analysis detected CIR mRNA at similar levels in human islets and exocrine pancreas, while the abundance of GIRK2 mRNA in the two tissues was insufficient for detection by this method. Using competitive reverse-transcription polymerase chain reaction, CIR was found to be present at higher levels than GIRK2 mRNA in native purified beta-cells. Xenopus oocytes injected with M2 muscarinic receptor (M2) plus either GIRK2 or CIR cRNA expressed only very small carbachol-induced currents, while co-injection of CIR plus GIRK2 along with M2 resulted in expression of carbachol-activated strong inwardly rectifying currents. Activators of KATP channels failed to elicit currents in the presence or absence of co-expressed sulfonylurea receptor. These results show that two components of islet cell KIR channels, CIR and GIRK2, may interact to form heteromeric G-protein-activated inwardly rectifying K+ channels that do not possess the typical properties of KATP channels.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
270
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
26086-91
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7592809-Amino Acid Sequence, pubmed-meshheading:7592809-Animals, pubmed-meshheading:7592809-Base Sequence, pubmed-meshheading:7592809-Cloning, Molecular, pubmed-meshheading:7592809-DNA Primers, pubmed-meshheading:7592809-Female, pubmed-meshheading:7592809-GTP-Binding Proteins, pubmed-meshheading:7592809-Gene Expression, pubmed-meshheading:7592809-Gene Library, pubmed-meshheading:7592809-Humans, pubmed-meshheading:7592809-Insulinoma, pubmed-meshheading:7592809-Islets of Langerhans, pubmed-meshheading:7592809-Kinetics, pubmed-meshheading:7592809-Macromolecular Substances, pubmed-meshheading:7592809-Membrane Potentials, pubmed-meshheading:7592809-Mice, pubmed-meshheading:7592809-Molecular Sequence Data, pubmed-meshheading:7592809-Oocytes, pubmed-meshheading:7592809-Organ Specificity, pubmed-meshheading:7592809-Pancreatic Neoplasms, pubmed-meshheading:7592809-Polymerase Chain Reaction, pubmed-meshheading:7592809-Potassium Channels, pubmed-meshheading:7592809-Potassium Channels, Inwardly Rectifying, pubmed-meshheading:7592809-Rats, pubmed-meshheading:7592809-Rats, Wistar, pubmed-meshheading:7592809-Recombinant Proteins, pubmed-meshheading:7592809-Sequence Homology, Amino Acid, pubmed-meshheading:7592809-Xenopus
pubmed:year
1995
pubmed:articleTitle
Pancreatic islet cells express a family of inwardly rectifying K+ channel subunits which interact to form G-protein-activated channels.
pubmed:affiliation
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't