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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1995-12-12
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pubmed:abstractText |
A fraction of activated CD8+ T cells expresses CD40 ligand (CD40L), a molecule that plays a key role in T cell-dependent B cell stimulation. CD8+ T cell clones were examined for CD40L expression and for their capacity to allow the growth and differentiation of B cells, upon activation with immobilized anti-CD3. According to CD40L expression, CD8+ clones could be grouped into three subsets. CD8+ T cell clones expressing high levels of CD40L (> or = 80% CD40L+ cells) were equivalent to CD4+ T cell clones with regard to induction of tonsil B cell proliferation and immunoglobulin (Ig) production, provided the combination of interleukin (IL)-2 and IL-10 was added to cultures. CD8+ T cell clones, with intermediate levels of CD40L expression (10 to 30% CD40L+ cells), also stimulated B cell proliferation and Ig secretion with IL-2 and IL-10. B cell responses induced by these CD8+ T cell clones were neutralized by blocking monoclonal antibodies specific for either CD40L or CD40. By contrast, CD40L- T cell clones (< or = 5% CD40L+ cells), only induced marginal B cell responses even with IL-2 and IL-10. All three clone types were able to activate B cells as shown by up-regulation of CD25, CD80 and CD86 expression. A neutralizing anti-CD40L antibody indicated that T cell-dependent B cell activation was only partly dependent on CD40-CD40L interaction. These CD40L- clones had no inhibitory effects on B cell proliferation induced by CD40L-expressing CD8+ T cell clones. Taken together, these results indicate that CD8+ T cells can induce B cell growth and differentiation in a CD40L-CD40-dependent fashion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/CD40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Dipeptidyl Peptidase 4,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogens,
http://linkedlifedata.com/resource/pubmed/chemical/Phytohemagglutinins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2972-7
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:7589100-Animals,
pubmed-meshheading:7589100-Antibody Formation,
pubmed-meshheading:7589100-Antigens, CD80,
pubmed-meshheading:7589100-B-Lymphocytes,
pubmed-meshheading:7589100-CD40 Ligand,
pubmed-meshheading:7589100-CD8-Positive T-Lymphocytes,
pubmed-meshheading:7589100-Cell Differentiation,
pubmed-meshheading:7589100-Cell Division,
pubmed-meshheading:7589100-Cells, Cultured,
pubmed-meshheading:7589100-Clone Cells,
pubmed-meshheading:7589100-Dipeptidyl Peptidase 4,
pubmed-meshheading:7589100-Flow Cytometry,
pubmed-meshheading:7589100-Humans,
pubmed-meshheading:7589100-Interleukin-10,
pubmed-meshheading:7589100-Interleukin-2,
pubmed-meshheading:7589100-L Cells (Cell Line),
pubmed-meshheading:7589100-Lymphocyte Activation,
pubmed-meshheading:7589100-Lymphocyte Cooperation,
pubmed-meshheading:7589100-Membrane Glycoproteins,
pubmed-meshheading:7589100-Mice,
pubmed-meshheading:7589100-Mitogens,
pubmed-meshheading:7589100-Palatine Tonsil,
pubmed-meshheading:7589100-Phytohemagglutinins,
pubmed-meshheading:7589100-Receptors, Interleukin-2,
pubmed-meshheading:7589100-T-Lymphocyte Subsets,
pubmed-meshheading:7589100-Transfection,
pubmed-meshheading:7589100-Up-Regulation
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pubmed:year |
1995
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pubmed:articleTitle |
CD40 ligand-positive CD8+ T cell clones allow B cell growth and differentiation.
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pubmed:affiliation |
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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