Linked Life Data

7587304

Source:http://linkedlifedata.com/resource/pubmed/id/7587304

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1995-12-14
pubmed:abstractText
The purpose of this experiment was to study in vivo the dynamic behavior of the lymphocyte in the retinal circulation. We developed a new technique capable of visualization of lymphocyte motion in the retinal and choroidal vessels using a rat model. Live cells freshly removed on a donor animal were labeled by a simple method using fluorescein isothiocyanate. Labeled cells were injected systemically into another animal. Retinal images were reconstituted on a video screen with a scanning laser ophthalmoscope (SLO) utilizing the argon green laser excitation wavelength (514.5 nm) to detect cell fluorescence. Lymphocytes were clearly seen and followed in the retinal vessels. Some slowed down in the capillary system, or even stopped for a few seconds, or were definitively caught in it. Labeled cells remained visible after circulating several times. A method was developed for in vivo visualization of lymphocytes in the retinal circulation. This method has the potential for application in the study of lymphocyte cell behavior under physiological as well as pathological conditions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0271-3683
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
579-84
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Study of lymphocyte dynamics in the ocular circulation: technique of labeling cells.
pubmed:affiliation
LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112-2234, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.