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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-12-28
|
| pubmed:abstractText |
The aim of this study was to determine whether the cytokine macrophage inflammatory protein-1 beta (MIP-1 beta) is present and functionally active in the arthritic joint. We used immunoassays and bioassays to assess the presence and function of MIP-1 beta using samples obtained from 62 arthritic patients. MIP-1 beta levels were increased in synovial fluids (SFs) from patients with osteoarthritis (OA) (18.0 +/- 8.9 ng/ml) (SD) compared to patients with rheumatoid arthritis (RA) 6.1 +/- 2.9 ng/ml) or other forms of arthritis (10.4 +/- 7.0 ng/ml) (P < 0.05). Levels of OA SF MIP-1 beta were significantly greater than OA or normal serum levels of MIP-1 beta. Anti-MIP-1 beta neutralized 28% of the chemotactic activity for monocytes found in OA SFs. Isolated OA synovial tissue fibroblasts did not constitutively produce MIP-1 beta but could be induced to express this chemokine upon exposure to tumor necrosis factor-alpha, interleukin-1 beta, or lipopolysaccharide. Synovial tissue immunohistochemical staining revealed that the main immunopositive cells in OA were the lining cells as well as vascular smooth muscle and endothelial cells. A minority of macrophages were immunopositive as well. In this study, we identify MIP-1 beta as a unique cytokine increased in OA compared to RA SF. We conclude that MIP-1 beta may play a role in the ingress of monocytes into the OA joint.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL4,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monokines
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0090-1229
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
77
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
307-14
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7586741-Arthritis,
pubmed-meshheading:7586741-Arthritis, Rheumatoid,
pubmed-meshheading:7586741-Cells, Cultured,
pubmed-meshheading:7586741-Chemokine CCL4,
pubmed-meshheading:7586741-Chemokines,
pubmed-meshheading:7586741-Chemotaxis, Leukocyte,
pubmed-meshheading:7586741-Cytokines,
pubmed-meshheading:7586741-Endothelium, Vascular,
pubmed-meshheading:7586741-Fibroblasts,
pubmed-meshheading:7586741-Humans,
pubmed-meshheading:7586741-Immune Sera,
pubmed-meshheading:7586741-Immunoenzyme Techniques,
pubmed-meshheading:7586741-Macrophage Inflammatory Proteins,
pubmed-meshheading:7586741-Monocytes,
pubmed-meshheading:7586741-Monokines,
pubmed-meshheading:7586741-Muscle, Smooth, Vascular,
pubmed-meshheading:7586741-Osteoarthritis,
pubmed-meshheading:7586741-Synovial Fluid,
pubmed-meshheading:7586741-Synovial Membrane
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Macrophage inflammatory protein-1 beta: a C-C chemokine in osteoarthritis.
|
| pubmed:affiliation |
Departments of Medicine, Northwestern University Medical School, Chicago, Illinois, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|