Linked Life Data

7583311

Source:http://linkedlifedata.com/resource/pubmed/id/7583311

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1995-12-14
pubmed:abstractText
Preservation of fetal ventral mesencephalic (VM) dopaminergic tissue prior to transplantation has been hampered by the fact that the cells are vulnerable to mechanical and osmotic stress after storage. Previous quantitative studies have shown that cool storage in a so-called 'hibernation medium' prior to grafting, can be used safely for up to 2 days without morphological or functional losses [16,32] using standard transplantation techniques. In the present study on rat fetal VM tissue we have investigated (i) the accuracy of different vital stains (trypan blue exclusion and ethidium bromide stain) to predict in vivo viability of VM cell suspensions after grafting; (ii) the influence of different storage media (glucose-saline, HBSS, DMEM, CO2-independent medium and hibernation medium), temperatures (+4 degrees C or +21 degrees C) and preparations (cell suspension or intact pieces) on the viability scores and total number of cells in vitro; and (iii) the survival and functional effects of intrastriatally grafted VM tissue after preservation by cool storage for up to 12 days using a less traumatic microtransplantation technique. The results show that cool storage at +4 degrees C of intact VM pieces in hibernation medium gives the best in vitro viability scores. Microtransplantation of cell suspensions prepared from cool-stored VM tissue produced good survival of tyrosine hydroxylase (TH)-positive graft neurons for up to 8 days of storage, and functional compensation in the amphetamine-rotation test for up to 12 days of storage. The total yield of surviving TH-positive neurons was unchanged, compared to fresh grafts, after 5 and 8 days of storage, and only reduced by 48% in the grafts stored for 12 days prior to implantation. These findings highlight the potential usefulness of a combination of cool storage and microtransplantation techniques to be able to extend the preservation periods of VM tissue. Such procedures may ultimately help to increase the safety and flexibility in experimental and clinical studies on neural transplantation of dopaminergic neurons.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
687
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
22-34
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7583311-Animals, pubmed-meshheading:7583311-Cell Survival, pubmed-meshheading:7583311-Cell Transplantation, pubmed-meshheading:7583311-Cold Temperature, pubmed-meshheading:7583311-Coloring Agents, pubmed-meshheading:7583311-Culture Media, pubmed-meshheading:7583311-Ethidium, pubmed-meshheading:7583311-Female, pubmed-meshheading:7583311-Graft Survival, pubmed-meshheading:7583311-Immunohistochemistry, pubmed-meshheading:7583311-Mesencephalon, pubmed-meshheading:7583311-Neostriatum, pubmed-meshheading:7583311-Neurons, pubmed-meshheading:7583311-Rats, pubmed-meshheading:7583311-Rats, Sprague-Dawley, pubmed-meshheading:7583311-Stereotyped Behavior, pubmed-meshheading:7583311-Sympathectomy, Chemical, pubmed-meshheading:7583311-Tissue Preservation, pubmed-meshheading:7583311-Trypan Blue, pubmed-meshheading:7583311-Tyrosine 3-Monooxygenase
pubmed:year
1995
pubmed:articleTitle
Preservation of fetal ventral mesencephalic cells by cool storage: in-vitro viability and TH-positive neuron survival after microtransplantation to the striatum.
pubmed:affiliation
Department of Medical Cell Research, University of Lund, Sweden.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't