Linked Life Data

7579443

Source:http://linkedlifedata.com/resource/pubmed/id/7579443

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1995-11-27
pubmed:abstractText
STK, a new member of the hepatocyte growth factor receptor family, is the receptor for macrophage-stimulating protein (MSP), which acts on murine resident peritoneal macrophages. We established polyclonal and monoclonal antibodies against STK and characterized the structure of STK protein and STK expression on cells of the mononuclear phagocyte system. Western blotting showed that the STK transcript is translated into a single-chain precursor and then cleaved into a 165-kD disulfide-linked heterodimer composed of a 35-kD alpha-chain and a 144-kD beta-chain. Western blotting detected STK protein on resident peritoneal macrophages, a target of MSP, and showed that it was autophosphorylated in cells stimulated by MSP. By flow cytometric analysis using a monoclonal anti-STK antibody, we showed that STK protein is expressed on restricted macrophage populations such as resident peritoneal macrophages, but not on exudate peritoneal macrophages or mononuclear phagocytes of the bone marrow, peripheral blood, spleen, or alveoli. Resident peritoneal macrophages were classified into two fractions according to their reactivity with an anti-STK antibody and a marker antibody for macrophages: STKhigh-F4/80high cells and STKnegative-F4/80low cells. Acute exudative macrophages were all STKnegative-F4/80low, but they gradually became predominantly STKhigh-F4/80high several days after entrance into the peritoneal cavity. These results showed that after monocytes migrate into the peritoneal cavity, they undergo terminal differentiation in the peritoneal microenvironment. This is the first evidence of tissue-specific terminal differentiation of peritoneal macrophages, and this terminal differentiation can be characterized by the expression of STK receptor tyrosine kinase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3394-403
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7579443-Animals, pubmed-meshheading:7579443-Antibodies, Monoclonal, pubmed-meshheading:7579443-Biological Markers, pubmed-meshheading:7579443-Blotting, Western, pubmed-meshheading:7579443-Cell Differentiation, pubmed-meshheading:7579443-Enzyme Induction, pubmed-meshheading:7579443-Flow Cytometry, pubmed-meshheading:7579443-Growth Substances, pubmed-meshheading:7579443-Hepatocyte Growth Factor, pubmed-meshheading:7579443-Macrophages, Peritoneal, pubmed-meshheading:7579443-Mice, pubmed-meshheading:7579443-Mice, Inbred C57BL, pubmed-meshheading:7579443-Organ Specificity, pubmed-meshheading:7579443-Peroxidases, pubmed-meshheading:7579443-Phagocytosis, pubmed-meshheading:7579443-Phosphorylation, pubmed-meshheading:7579443-Protein Processing, Post-Translational, pubmed-meshheading:7579443-Proto-Oncogene Proteins, pubmed-meshheading:7579443-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:7579443-Receptors, Cell Surface
pubmed:year
1995
pubmed:articleTitle
Terminal differentiation of murine resident peritoneal macrophages is characterized by expression of the STK protein tyrosine kinase, a receptor for macrophage-stimulating protein.
pubmed:affiliation
Department of Cell Differentiation, Kumamoto University School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't