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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1995-12-18
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pubmed:abstractText |
A 5'-GCGAAAGC minihairpin structure was added to the 3'-end of an oligonucleotide substituted at the 5'-end by a manganese cationic porphyrin in order to enhance the 3'-exonuclease resistance of these cleaver-antisense molecules. The influence of this minihairpin on the 3'-exonuclease resistance, the binding affinity to a target ssDNA, and the cleaving efficiency of Mn-cationic porphyrin oligonucleotide conjugates was compared to that of the parent molecule without the 3'-hairpin. The results showed that the 3'-hairpin slightly decreased the binding affinity and consequently the cleaving efficiency of the conjugated molecule toward a target sequence, but the much higher nuclease resistance makes 3'-minihairpin-protected metalloporphyrin oligonucleotides good candidates as reactive antisense oligonucleotides for studies on cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1043-1802
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
466-72
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7578367-Base Sequence,
pubmed-meshheading:7578367-DNA, Single-Stranded,
pubmed-meshheading:7578367-Exodeoxyribonuclease V,
pubmed-meshheading:7578367-Exodeoxyribonucleases,
pubmed-meshheading:7578367-Indicators and Reagents,
pubmed-meshheading:7578367-Manganese,
pubmed-meshheading:7578367-Metalloporphyrins,
pubmed-meshheading:7578367-Molecular Sequence Data,
pubmed-meshheading:7578367-Nucleic Acid Conformation,
pubmed-meshheading:7578367-Nucleic Acid Denaturation,
pubmed-meshheading:7578367-Oligodeoxyribonucleotides,
pubmed-meshheading:7578367-Oligonucleotides, Antisense,
pubmed-meshheading:7578367-Substrate Specificity,
pubmed-meshheading:7578367-Thermodynamics
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pubmed:articleTitle |
Preparation and nuclease activity of hybrid "metallotris(methylpyridinium)porphyrin oligonucleotide" molecules having a 3'-loop for protection against 3'-exonucleases.
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pubmed:affiliation |
Laboratoire de Chimie de Coordination du CNRS, Toulouse, France.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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