Linked Life Data

7578222

Source:http://linkedlifedata.com/resource/pubmed/id/7578222

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-12-5
pubmed:databankReference
pubmed:abstractText
A 16 kDa polypeptide chain (chain E) was isolated from the giant extracellular chlorocruorin from the polychaete Sabellastarte indica by reverse-phase chromatography, and the N-terminal 19 amino-acid residues was determined by an automated protein sequencer. The cDNA of Sabellastarte chain E was amplified by polymerase chain reaction (PCR), and the complete nucleotide sequence of 1205 bp was determined. The open reading frame is 498 nucleotides in length and encodes a protein with 165 amino-acid residues. Comparison of the cDNA-derived amino-acid sequence with the protein sequence shows that Sabellastarte chain E has a signal peptide of 16 residues at the N-terminus, the mature protein consisting of 149 amino-acid residues with a calculated molecular mass of 16636 Da. The amino-acid sequence of Sabellastarte chain E shows 42-49% sequence identity with the corresponding chains of the giant hemoglobins from Tylorrhynchus (polychaete, Annelida), Lumbricus (oligochaete, Annelida), Lamellibrachia (Vestimentifera) and Oligobrachia (Pogonophora). Thus, we conclude that chlorocruorin with chlorocruorohaem falls into the 'hemoglobin/myoglobin family'. This is the first complete sequence of a globin polypeptide chain of a chlorocruorin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
1252
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
189-93
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Primary structure of a constituent polypeptide chain of the chlorocruorin from Sabellastarte indica.
pubmed:affiliation
Department of Biology, Faculty of Science, Kochi University, Japan.
pubmed:publicationType
Journal Article, Comparative Study