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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1995-12-26
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pubmed:abstractText |
An inflammatory reaction is regularly noticed in irradiated tissues following photodynamic therapy (PDT). This observation is potentially associated with leucocyte-mediated tissue damage, which might further contribute to the tumoricidal effect of this therapy. The objective of our study was to investigate the effects of PDT on leucocyte-endothelium interaction in the microvasculature of tumours and normal tissue. Experiments were performed in the dorsal skinfold chamber preparation of Syrian golden hamsters bearing amelanotic melanoma A-Mel-3. The photosensitiser. Photofrin (5 mg kg-1 i.v.) was injected 24 h before laser irradiation (630 nm, 100 mW cm-2, 10 J cm-2 or 100 J cm-2). Post-capillary confluent venules (diameter 15-40 microns) of subcutaneous (s.c.) tissue or the amelanotic melanoma A-Mel-3 were observed by intravital microscopy before, 5, 30, 60 and 180 min after laser irradiation and recorded for off-line analysis. Before treatment, the number of adherent leucocytes in tumour vessels was only 22% of the number observed in vessels of s.c. tissue (P < 0.01). The maximum increase in adhering leucocytes was observed in post-capillary venules of s.c. tissue 1 h after PDT (P < 0.01). In contrast, enhanced leucocyte-endothelium interaction was missing in tumour vessels and in control groups. These results indicate that the tumour destruction observed after PDT is not mediated by leucocyte-endothelium interaction in the tumour. Induction of leucocyte adhesion in the PDT-treated normal tissue suggests a contribution to the peritumoral inflammatory response. Different maturational status or biochemical properties of tumour microvascular endothelium may explain the lack of leucocyte adherence upon PDT.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-13700922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-1387584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-1603846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-1638539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-1704376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-1715568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-1886985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-1887915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-1934331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-1997196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-2146455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-2293654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-2531034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-2539390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-2772144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-2857188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-2964665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-2969280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-3441511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-3638187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-3819468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-4624453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-6150958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-634160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-6678842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-7003665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-7214358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-7507411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-7690867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-8064187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-8142170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-8180019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-8194873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7577457-8234474
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0007-0920
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1125-30
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7577457-Animals,
pubmed-meshheading:7577457-Cell Adhesion,
pubmed-meshheading:7577457-Cell Movement,
pubmed-meshheading:7577457-Cricetinae,
pubmed-meshheading:7577457-Endothelium, Vascular,
pubmed-meshheading:7577457-Hematoporphyrin Derivative,
pubmed-meshheading:7577457-Hematoporphyrin Photoradiation,
pubmed-meshheading:7577457-Inflammation,
pubmed-meshheading:7577457-Lasers,
pubmed-meshheading:7577457-Leukocytes,
pubmed-meshheading:7577457-Male,
pubmed-meshheading:7577457-Melanoma,
pubmed-meshheading:7577457-Muscle, Skeletal,
pubmed-meshheading:7577457-Neoplasm Transplantation,
pubmed-meshheading:7577457-Oxygen,
pubmed-meshheading:7577457-Photosensitizing Agents,
pubmed-meshheading:7577457-Skin Window Technique,
pubmed-meshheading:7577457-Venules
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pubmed:year |
1995
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pubmed:articleTitle |
Effects of photodynamic therapy on leucocyte-endothelium interaction: differences between normal and tumour tissue.
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pubmed:affiliation |
Institute for Surgical Reserch, Klinikum Grosshadern, Ludwig-Maximilians-University, Munich, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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