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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1995-11-13
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pubmed:databankReference |
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pubmed:abstractText |
The role of flavivirus-cross-reactive T lymphocytes in recovery from and pathogenesis of flavivirus infections is not known. In the present paper, we have defined a flavivirus-cross-reactive epitope recognized by two CD4+ CD8- cytotoxic T lymphocyte (CTL) clones, JK4 and JK43. The T cell clones were established from the peripheral blood T lymphocytes of a dengue-4-immune donor, using a limiting-dilution method with dengue-4 antigen. These two T cell clones were cross-reactive for dengue virus types 1, 2, 3 and 4, yellow fever virus and West Nile virus, and recognized NS3 protein. The smallest synthetic peptide recognized by these T cell clones was an identical 9 amino acid peptide which contains amino acids 146 to 154 (VIGLYGNGV) of dengue-4 NS3. HLA-DR15 was the restriction allele for recognition of this epitope by JK4 and JK43. JK4 and JK43 both used T cell receptor V alpha 8, but JK4 used V beta 8 and JK43 used V beta 2. This result indicates that this epitope is recognized by two flavivirus-cross-reactive CD4+ T cell clones which originated from different T cells in vivo.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/NS3 protein, flavivirus,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Nonstructural Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76 ( Pt 9)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2243-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7561761-Amino Acid Sequence,
pubmed-meshheading:7561761-Animals,
pubmed-meshheading:7561761-Antigens, CD8,
pubmed-meshheading:7561761-Base Sequence,
pubmed-meshheading:7561761-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7561761-Cercopithecus aethiops,
pubmed-meshheading:7561761-Clone Cells,
pubmed-meshheading:7561761-Cross Reactions,
pubmed-meshheading:7561761-DNA,
pubmed-meshheading:7561761-Epitope Mapping,
pubmed-meshheading:7561761-Flavivirus,
pubmed-meshheading:7561761-HLA-DR Antigens,
pubmed-meshheading:7561761-Humans,
pubmed-meshheading:7561761-Molecular Sequence Data,
pubmed-meshheading:7561761-RNA Helicases,
pubmed-meshheading:7561761-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:7561761-Sequence Homology, Amino Acid,
pubmed-meshheading:7561761-Serine Endopeptidases,
pubmed-meshheading:7561761-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:7561761-Vero Cells,
pubmed-meshheading:7561761-Viral Nonstructural Proteins
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pubmed:year |
1995
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pubmed:articleTitle |
Flavivirus-cross-reactive, HLA-DR15-restricted epitope on NS3 recognized by human CD4+ CD8- cytotoxic T lymphocyte clones.
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pubmed:affiliation |
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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