Linked Life Data

7557433

Source:http://linkedlifedata.com/resource/pubmed/id/7557433

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-11-6
pubmed:databankReference
pubmed:abstractText
A novel baculovirus expression vector (pBSV-8His) directs secretion of recombinant proteins into the culture medium of infected insect cells. By providing a vector-encoded signal peptide upstream from a multiple cloning site, the product of the inserted cDNA is directed to the secretory pathway. In addition, a C-terminal His-tag allows convenient purification of the native protein directly from the culture medium in less than 5 h. The His-tag can be cleaved off the purified protein by utilizing an enterokinase cleavage site located directly N-terminal to the His sequence. By insertion of a coding sequence representing the human complement regulatory factor H-like (FHL-1) plasma protein into pBSV-8His, a high level of protein synthesis was demonstrated (9 micrograms/ml). The high level of production and the ease with which native protein can be purified almost to homogeneity, makes pBSV-8His particularly suitable for protein synthesis and purification. The combination of a vector-encoded signal peptide and a C-terminal His-tag allows production and direct purification of individual domains of secretory proteins in eukaryotic cells. This feature makes this novel vector extremely useful for structure function analyses of secretory proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
162
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
225-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
The baculovirus expression vector pBSV-8His directs secretion of histidine-tagged proteins.
pubmed:affiliation
Department of Molecular Biology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't