Linked Life Data

7554938

Source:http://linkedlifedata.com/resource/pubmed/id/7554938

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-4
pubmed:dateCreated
1995-11-6
pubmed:abstractText
This study describes the immunolocalization of actin, cytokeratins and vimentin during differentiation of the dental papilla in the rat. Incisors and first molars were sectioned from mandibles of Wistar rats from embryonic day (E)-14 to (E)-21 and weeks 1, 2, 3, 12 and 104 after birth, fixed in 90% alcohol, decalcified in EDTA, infiltrated with 5% sucrose, frozen in dry ice, and cryosectioned at 10 microns. The sections were immunolabelled using indirect immunofluorescence with a panel of monoclonal antibodies and FITC-phalloidin for F-actin localization. F-actin was present in follicular mesenchyme and odontoblast processes. Vimentin labelled dental papilla fibroblasts, differentiating, functional (secretory) and aged odontoblasts. Vimentin was uniformly localized in the cytoplasm of pre-odontoblasts but was redistributed to the apical pole of these cells during polarization. Of the cytokeratins, only cytokeratin 19 was found in differentiating odontoblasts. It was not present in dental papilla fibroblasts, functional or aged odontoblasts. These results suggest that actin and the redistribution of vimentin may be involved in odontoblast differentiation and odontoblast process formation/support and that these events may be preceded by the expression of cytokeratin 19.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-8207
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Cytoskeleton of the mesenchymal cells of the rat dental papilla and dental pulp.
pubmed:affiliation
School of Molecular and Medical Biosciences (Anatomy Unit), Cardiff.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't