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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2 Pt 2
|
| pubmed:dateCreated |
1995-9-22
|
| pubmed:abstractText |
Oligosyndactyly mice (ROP Os/+) are a radiation-induced mutant strain with reduced glomerular number and increased glomerular size. We found that they develop glomerulosclerosis. At 3 mo, ROP Os/+ mice had diffuse mesangial expansion by light microscopy, whereas their +/+ littermates did not. Electron microscopic morphometry revealed a twofold increase in mesangial areas but no changes in the thickness of glomerular basal laminae. Mean glomerular volume was increased 1.8-fold. Cell number and thymidine labeling index were increased 1.3- and 2.4-fold, respectively. The amount of glomerular type IV collagen and tenascin but not laminin was increased by immunofluorescence microscopy. mRNA levels in microdissected glomeruli were measured by competetive reverse transcription-polymerase chain reaction and corrected for cell number. alpha 1-Chain type IV collagen and tenascin mRNAs were increased 3.2-fold and 1.8-fold, whereas laminin B1 mRNA levels were not. The levels of 72-kDa collagenase mRNA were increased 1.6-fold. Transforming growth factor-beta 1 mRNA levels were elevated 1.8-fold, but platelet-derived growth factor-B mRNA levels remained normal. This is the first analysis of glomerular molecular and cellular changes in a model of congenital nephron reduction.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules, Neuronal,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Collagenases,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tenascin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
269
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
F266-73
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7544540-Actins,
pubmed-meshheading:7544540-Animals,
pubmed-meshheading:7544540-Cell Adhesion Molecules, Neuronal,
pubmed-meshheading:7544540-Collagen,
pubmed-meshheading:7544540-Collagenases,
pubmed-meshheading:7544540-Extracellular Matrix Proteins,
pubmed-meshheading:7544540-Female,
pubmed-meshheading:7544540-Fluorescent Antibody Technique,
pubmed-meshheading:7544540-Glomerulosclerosis, Focal Segmental,
pubmed-meshheading:7544540-Kidney,
pubmed-meshheading:7544540-Kidney Glomerulus,
pubmed-meshheading:7544540-Mice,
pubmed-meshheading:7544540-Mice, Mutant Strains,
pubmed-meshheading:7544540-Microscopy, Fluorescence,
pubmed-meshheading:7544540-Nephrons,
pubmed-meshheading:7544540-Organ Size,
pubmed-meshheading:7544540-RNA, Messenger,
pubmed-meshheading:7544540-Tenascin
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Molecular analysis of spontaneous glomerulosclerosis in Os/+ mice, a model with reduced nephron mass.
|
| pubmed:affiliation |
Renal Cell Biology Section, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892-1268, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|