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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
31
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pubmed:dateCreated |
1995-9-7
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pubmed:abstractText |
Binding studies with a CD6 immunoglobulin fusion protein (CD6 Rg) resulted in the identification and cloning of a CD6 ligand. This ligand was found to be a member of the immunoglobulin supergene family and was named ALCAM (activated leukocyte cell adhesion molecule). Cell adhesion assays showed that CD6-ALCAM interactions mediate thymocyte-thymic epithelium cell binding. ALCAM is also expressed by activated leukocytes and neurons and may be involved in interactions between T cells and activated leukocytes and between cells of the immune and nervous systems, respectively. Herein we describe the preparation of domain-specific murine CD6 Rg fusion proteins and show that the membrane-proximal SRCR (scavenger receptor cysteine-rich) domain of CD6 contains the ALCAM binding site. We also show that mAbs which bind to this domain preferentially block CD6-ALCAM binding. These results demonstrate that the membrane-proximal SRCR domain of CD6 is necessary for CD6 binding to ALCAM and provide the first direct evidence for the interaction of an SRCR domain with a ligand.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Activated-Leukocyte Cell Adhesion...,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD6 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lipoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Scavenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Scarb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Scavenger Receptors, Class B
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
18187-90
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:7543097-Activated-Leukocyte Cell Adhesion Molecule,
pubmed-meshheading:7543097-Antibodies, Monoclonal,
pubmed-meshheading:7543097-Antibody Specificity,
pubmed-meshheading:7543097-Antigens, CD,
pubmed-meshheading:7543097-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:7543097-Binding Sites,
pubmed-meshheading:7543097-Cell Adhesion Molecules,
pubmed-meshheading:7543097-Epitopes,
pubmed-meshheading:7543097-Glycoproteins,
pubmed-meshheading:7543097-Humans,
pubmed-meshheading:7543097-Membrane Proteins,
pubmed-meshheading:7543097-Peptide Fragments,
pubmed-meshheading:7543097-Protein Binding,
pubmed-meshheading:7543097-Receptors, Immunologic,
pubmed-meshheading:7543097-Receptors, Lipoprotein,
pubmed-meshheading:7543097-Receptors, Scavenger,
pubmed-meshheading:7543097-Recombinant Fusion Proteins,
pubmed-meshheading:7543097-Scavenger Receptors, Class B,
pubmed-meshheading:7543097-Structure-Activity Relationship
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pubmed:year |
1995
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pubmed:articleTitle |
The membrane-proximal scavenger receptor cysteine-rich domain of CD6 contains the activated leukocyte cell adhesion molecule binding site.
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pubmed:affiliation |
Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121, USA.
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pubmed:publicationType |
Journal Article
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