Linked Life Data

7541674

Source:http://linkedlifedata.com/resource/pubmed/id/7541674

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-8-15
pubmed:abstractText
Fluorescence in situ hybridization (FISH) was used to discriminate between benign and malignant cells in sorted populations of chronic myelogenous leukemia (CML) marrow. FISH has the advantage of allowing for a cell by cell analysis of the breakpoint cluster region (BCR) gene rearrangement immediately after flow sorting in nondividing G0/G1 cells that are potentially transcriptionally inactive. We initially selected CD34+ cells with very low expression of the activation antigen CD38 as a candidate phenotype for an immature and hypothetically more benign cell population, but found no enrichment for Ph negativity in that subtype. In five CML samples, 55% +/- 3.3% (mean +/- SE) of CD34+/CD38hi cells had the BCR gene rearrangement, similar to 57% +/- 3.7% seen in the CD34+/CD38lo population. In contrast, subsequent experiments (n = 4) determined that the CD34+/HLA-DRlo population in CML marrow does contain an increased proportion of benign cells: 15% +/- 1% of the CD34+/DRlo cells were BCR rearranged, compared with 52% +/- 5.8% of the CD34+/DRhi cells (P = .001). Our results indicate that benign progenitors in CML are enriched within the CD34+ cells with low DR antigen expression, but not low CD38 expression. One possible interpretation of these observations is that low CD38 antigen expression is not as useful as low HLA-DR expression for isolating immature cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
737-43
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7541674-ADP-ribosyl Cyclase, pubmed-meshheading:7541674-Adult, pubmed-meshheading:7541674-Antigens, CD, pubmed-meshheading:7541674-Antigens, CD34, pubmed-meshheading:7541674-Antigens, CD38, pubmed-meshheading:7541674-Antigens, Differentiation, pubmed-meshheading:7541674-Bone Marrow, pubmed-meshheading:7541674-Female, pubmed-meshheading:7541674-Fusion Proteins, bcr-abl, pubmed-meshheading:7541674-Gene Rearrangement, pubmed-meshheading:7541674-HLA-DR Antigens, pubmed-meshheading:7541674-Hematopoietic Stem Cells, pubmed-meshheading:7541674-Humans, pubmed-meshheading:7541674-In Situ Hybridization, Fluorescence, pubmed-meshheading:7541674-Interphase, pubmed-meshheading:7541674-Leukemia, Myelogenous, Chronic, BCR-ABL Positive, pubmed-meshheading:7541674-Male, pubmed-meshheading:7541674-Membrane Glycoproteins, pubmed-meshheading:7541674-Middle Aged, pubmed-meshheading:7541674-N-Glycosyl Hydrolases
pubmed:year
1995
pubmed:articleTitle
Benign marrow progenitors are enriched in the CD34+/HLA-DRlo population but not in the CD34+/CD38lo population in chronic myeloid leukemia: an analysis using interphase fluorescence in situ hybridization.
pubmed:affiliation
Fred Hutchinson Cancer Research Center, Seattle, WA 98104-2092, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't