Linked Life Data

7537990

Source:http://linkedlifedata.com/resource/pubmed/id/7537990

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1995-6-12
pubmed:abstractText
Bleomycin (BLM), a currently used anticancer drug, crosses the plasma membrane poorly. Electropermeabilization allows a defined number of BLM molecules to enter directly into the cell cytoplasm. Such a procedure has revealed that BLM is endowed with a very high intrinsic cytotoxicity. Here we show that when several million molecules of BLM are internalized, morphological changes identical to those usually associated with apoptosis are observed as well as very rapid DNA fragmentation into oligonucleosomal-sized fragments. We demonstrate that this fragmentation, which occurs within a few seconds after BLM internalization, is consistent with a direct internucleosomal cleavage of chromatin by BLM. This result reinforces the importance of DNA digestion as an early and essential step in the morphological changes associated with apoptosis. Our study also demonstrates that BLM cytotoxicity is actually due to the DNA double strand breaks generated by this drug.
pubmed:language
fre
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0007-4551
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1043-9
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
[Bleomycin is an antineoplastic agent capable of mimicking apoptosis].
pubmed:affiliation
Laboratoire de biochimie-enzymologie, URA 147 CNRS, institut Gustave-Roussy, Villejuif, France.
pubmed:publicationType
Journal Article, English Abstract