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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-6-5
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pubmed:abstractText |
We have found that in the primary culture of rat hepatocytes, the potent mitogenic activity of native FGF-1 is independent of heparin. The well-established characteristic of FGF-1 as a heparin-dependent mitogen is confirmed by human umbilical vein endothelial cells using the same preparation of FGF-1. Cross-linking experiments reveal that binding of FGF-1 to the hepatocyte cell surface receptors can be accomplished in the absence of exogenous heparin, in contrast to human endothelial cells for which it remains as a limiting factor. For both cell types, however, it is demonstrated that either endogenous or exogenous heparan sulfate/heparin moieties are essential for FGF-1 to establish receptor binding and mitogen action. Thus, the results suggest that hepatocytes harbor cell surface heparan sulfate moieties that are fully capable of utilizing FGF-1 in the environment. These results raise the possibility that FGF-1 is of differential potency for different cell types according to the nature and/or quantity of cell surface heparan sulfate moieties in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0014-4827
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
218
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
233-40
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7537691-Animals,
pubmed-meshheading:7537691-Brain,
pubmed-meshheading:7537691-Cattle,
pubmed-meshheading:7537691-Cell Membrane,
pubmed-meshheading:7537691-Cells, Cultured,
pubmed-meshheading:7537691-DNA,
pubmed-meshheading:7537691-Endothelium, Vascular,
pubmed-meshheading:7537691-Female,
pubmed-meshheading:7537691-Fibroblast Growth Factor 1,
pubmed-meshheading:7537691-Heparin,
pubmed-meshheading:7537691-Hepatocyte Growth Factor,
pubmed-meshheading:7537691-Humans,
pubmed-meshheading:7537691-Kinetics,
pubmed-meshheading:7537691-Liver,
pubmed-meshheading:7537691-Mitotic Index,
pubmed-meshheading:7537691-Polysaccharide-Lyases,
pubmed-meshheading:7537691-Rats,
pubmed-meshheading:7537691-Rats, Wistar,
pubmed-meshheading:7537691-Receptors, Fibroblast Growth Factor,
pubmed-meshheading:7537691-Umbilical Veins
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pubmed:year |
1995
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pubmed:articleTitle |
FGF-1 is a heparin-independent mitogen for rat hepatocytes.
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pubmed:affiliation |
Cell Biology Laboratory, National Institute of Bioscience and Human Technology, Ibaraki, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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