Linked Life Data

7535416

Source:http://linkedlifedata.com/resource/pubmed/id/7535416

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1995-5-3
pubmed:abstractText
The B-raf/c-Rmil proto-oncogene belongs to the raf/mil family of serine/threonine protein kinases. It encodes multiple protein isoforms previously shown to be expressed predominantly in neural tissues. We report here that B-Raf proteins of 95 and 72 kDa are also expressed in various human and murine hematopoietic cell lines. Their relative level of expression is variable depending on the cell line examined. The highest level of expression of p95B-raf was found in UT-7 cells, a human pluripotent cell line established from a patient with a megakaryoblastic leukemia. These cells are able to differentiate toward erythroid or myeloid lineage phenotypes in presence of erythropoietin (EPO) or granulocyte-macrophage colony-stimulating factor (GM-CSF) respectively. We show that treatment of UT-7 cells with EPO, GM-CSF or stem cell factor (SCF) rapidly induces phosphorylation of p95B-raf as indicated by a shift of electrophoretic mobility. This increase in phosphorylation is correlated with a three-fold increase of B-Raf kinase activity. B-Raf activation also increases in a dose-dependent manner in response to EPO and GM-CSF. We also show that both p95B-raf and p72B-raf can be activated by IL-3 in murine BAF-3 pro-B cells and by anti-CD3 in human Jurkat cells, respectively. These observations provide the first evidence that the B-Raf kinase is involved in signal transduction pathways regulating proliferation and differentiation of hematopoietic cells of both myeloid and lymphoid lineages.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
10
pubmed:geneSymbol
c-mil, c-raf
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1159-65
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7535416-Animals, pubmed-meshheading:7535416-Cell Line, pubmed-meshheading:7535416-Enzyme Activation, pubmed-meshheading:7535416-Erythropoietin, pubmed-meshheading:7535416-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:7535416-Hematopoietic Cell Growth Factors, pubmed-meshheading:7535416-Hematopoietic Stem Cells, pubmed-meshheading:7535416-Humans, pubmed-meshheading:7535416-Isoenzymes, pubmed-meshheading:7535416-Leukemia, pubmed-meshheading:7535416-Mice, pubmed-meshheading:7535416-Phosphorylation, pubmed-meshheading:7535416-Protein-Serine-Threonine Kinases, pubmed-meshheading:7535416-Proto-Oncogene Proteins, pubmed-meshheading:7535416-Proto-Oncogene Proteins c-raf, pubmed-meshheading:7535416-Stem Cell Factor, pubmed-meshheading:7535416-Tumor Cells, Cultured
pubmed:year
1995
pubmed:articleTitle
Expression and activation of B-Raf kinase isoforms in human and murine leukemia cell lines.
pubmed:affiliation
Unité de Recherche Associée 1443 du CNRS, Institut Curie, Centre Universitaire, Orsay, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't