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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-3-21
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pubmed:abstractText |
1. The profiles of cyclo-oxygenase (COX) and nitric oxide synthase (NOS) isoforms were determined in the rat carrageenin-induced pleurisy model of acute inflammation. 2. The enzymes were assessed in peripheral blood leucocyte (PBL) cell pellets taken from untreated animals and at 2, 6 and 24 h after injection of the irritant in pleural exudate cell pellets and lung homogenates. 3. COX activity was assessed by the generation of prostacyclin (PGI2, measured as the stable metabolite, 6-keto prostaglandin F1 alpha) and prostaglandin E2 (PGE2). Western blot analysis and immunohistochemistry were also carried out. 4. NOS activity was based on the conversion of [3H]-L-arginine to [3H]-L-citrulline in the presence (total NOS activity) or absence of Ca2+ (inducible NOS; iNOS). 5. Peripheral blood leucocyte samples contained low levels of COX activity. In pleural exudate cell pellets, COX activity peaked at 2 to 6 h after injection of the carrageenin. At 24 h, COX activity was significantly reduced. 6. Western blot analysis demonstrated that the inducible isoform of COX (COX-2), was the predominant enzyme at all time points. Low levels of COX-2 were seen in PBLs. In pleural exudate cell pellets maximal COX-2 protein levels were seen at 2 h. 7. Immunohistochemistry confirmed the findings of Western blot studies. Approximately 10% of polymorphonuclear neutrophils (PMNs) in PBLs from untreated animals were immunopositive for COX-2. In cell pellet smears from carrageenin-induced pleurisy taken 2 h after injection of the irritant, PMNs were also the major source of COX-2 immunoreactivity. A small proportion of macrophages and mesothelial cells were also immunolabelled for COX-2.8. Low levels of NOS activity were seen in PBLs. In pleural exudates NOS activity was maximum at 6 h and greatly reduced by 24 h. This activity was solely attributable to iNOS.9. The present results illustrated a similar profile of COX and NOS activity in the carrageenin-induced pleurisy model of acute inflammation. It was demonstrated that COX-2 and iNOS were the predominant isoforms of their respective enzymes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-1648737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-1701676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-1717286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-1720618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-1852778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-2106348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-2541713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-2549965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-2819872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-3111928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-3242600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-3495737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-4760142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-5284360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-6409932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-6415107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-6952258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-7268440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-7504017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-7510883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-8242242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-8265610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-8349699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7532080-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
113
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
693-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7532080-6-Ketoprostaglandin F1 alpha,
pubmed-meshheading:7532080-Amino Acid Oxidoreductases,
pubmed-meshheading:7532080-Animals,
pubmed-meshheading:7532080-Blotting, Western,
pubmed-meshheading:7532080-Carrageenan,
pubmed-meshheading:7532080-Dinoprostone,
pubmed-meshheading:7532080-Immunohistochemistry,
pubmed-meshheading:7532080-Isoenzymes,
pubmed-meshheading:7532080-Male,
pubmed-meshheading:7532080-Nitric Oxide Synthase,
pubmed-meshheading:7532080-Pleurisy,
pubmed-meshheading:7532080-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:7532080-Rats,
pubmed-meshheading:7532080-Rats, Wistar
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pubmed:year |
1994
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pubmed:articleTitle |
Cyclo-oxygenase and nitric oxide synthase isoforms in rat carrageenin-induced pleurisy.
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pubmed:affiliation |
Department of Experimental Pathology, William Harvey Research Institute, St. Bartholomew's Hospital Medical College, London.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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