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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1995-3-23
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pubmed:abstractText |
Partially desialylated ovine submaxillary mucin plus an immunological adjuvant has been used by us to induce a humoral immune response to Tn antigen and sialylated Tn in colon cancer patients at risk of recurrence. Peripheral blood lymphocytes were purified from one of these patients with a high IgM titer reactive with Tn antigen transformed with Epstein-Barr virus and subsequently fused with a human-mouse heteromyeloma cell line, HMMA2.11TG/O. Clones were screened and subcloned using an enzyme-linked immunoassay and four stable IgM-secreting clones were tested for reactivity with a variety of natural and synthetic antigens, paraffin-embedded colon carcinomas and normal colonic mucosa to determine their specificity. Four human IgM monoclonal antibodies reactive predominantly with Tn antigen were produced and shown to react with a mucinous human colon carcinoma cell line, LS-174T, and with paraffin-embedded human colon carcinomas. We conclude that modified ovine submaxillary mucin is an effective vaccine for generating a humoral immune response directed to Tn antigen present on human colon cancer.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Tumor-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Mucins,
http://linkedlifedata.com/resource/pubmed/chemical/Sialic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Tn antigen
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pubmed:status |
MEDLINE
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pubmed:issn |
0956-960X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25-31
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7532023-Animals,
pubmed-meshheading:7532023-Antibodies, Monoclonal,
pubmed-meshheading:7532023-Antibodies, Neoplasm,
pubmed-meshheading:7532023-Antibody Specificity,
pubmed-meshheading:7532023-Antigens, Tumor-Associated, Carbohydrate,
pubmed-meshheading:7532023-Colonic Neoplasms,
pubmed-meshheading:7532023-Epitopes,
pubmed-meshheading:7532023-Humans,
pubmed-meshheading:7532023-Hybridomas,
pubmed-meshheading:7532023-Immunoglobulin M,
pubmed-meshheading:7532023-Immunohistochemistry,
pubmed-meshheading:7532023-Mice,
pubmed-meshheading:7532023-Mucins,
pubmed-meshheading:7532023-Sheep,
pubmed-meshheading:7532023-Sialic Acids,
pubmed-meshheading:7532023-Submandibular Gland,
pubmed-meshheading:7532023-Tumor Cells, Cultured,
pubmed-meshheading:7532023-Vaccination
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pubmed:year |
1994
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pubmed:articleTitle |
Anti-Tn human monoclonal antibodies generated following active immunization with partially desialylated ovine submaxillary mucin.
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pubmed:affiliation |
Montefiore Medical Center/Albert Einstein Cancer Center, Bronx, New York 10467.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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