Linked Life Data

7530038

Source:http://linkedlifedata.com/resource/pubmed/id/7530038

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1995-2-22
pubmed:abstractText
Development of the antigen-specific murine T cell response to immunization with keyhole limpet hemocyanin (KLH) in adjuvant has been monitored with direct limiting dilution analysis of CD4+ cells in draining lymph nodes (LN) and measurement of the cytokines produced by their clonal progeny. In vivo, the response to immunization suggested a major role for IL-4 and a minor role for IFN-gamma since IL-4 mRNA levels increased and IFN-gamma mRNA levels declined in LN over the first 3 days, and KLH-specific serum antibodies were mainly of IgG1 class with lower levels of IgE and IgG2a. Antigen-specific clonogenic cells were first detected in LN at day 4, at which time they comprised approximately 8% of the total CD4+ LN cell pool, declining to 1-2% from day 7 until at least 6 weeks after immunization. These clonogenic cells expressed high levels of surface CD44 (Pgp-1) both early and late in the in vivo response. Over the whole of the time span from day 4 to 6 weeks after immunization, most antigen-specific cells gave rise to clones that secreted IL-4 and a smaller proportion gave rise to IFN-gamma-secreting clones. By contrast, polyclonally activated CD4+ cells from untreated mice preferentially gave rise to clones with the converse cytokine profile. We conclude that a stable ratio of antigen-specific CD4+ cells committed to IL-4 or IFN-gamma synthesis is established within the first 4 days after KLH immunization and, contrary to prediction, does not evolve towards a more restricted cytokine profile during the primary response.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0953-8178
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1515-23
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7530038-Animals, pubmed-meshheading:7530038-Antigens, pubmed-meshheading:7530038-Antigens, CD44, pubmed-meshheading:7530038-Bordetella pertussis, pubmed-meshheading:7530038-CD4-Positive T-Lymphocytes, pubmed-meshheading:7530038-Carrier Proteins, pubmed-meshheading:7530038-Cells, Cultured, pubmed-meshheading:7530038-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:7530038-Female, pubmed-meshheading:7530038-Hemocyanin, pubmed-meshheading:7530038-Immunoglobulins, pubmed-meshheading:7530038-Interferon-gamma, pubmed-meshheading:7530038-Interleukin-4, pubmed-meshheading:7530038-Lymph Nodes, pubmed-meshheading:7530038-Mice, pubmed-meshheading:7530038-Mice, Inbred C57BL, pubmed-meshheading:7530038-RNA, Messenger, pubmed-meshheading:7530038-Receptors, Cell Surface, pubmed-meshheading:7530038-Receptors, Lymphocyte Homing
pubmed:year
1994
pubmed:articleTitle
Rapid establishment of a stable IL-4/IFN-gamma production profile in the antigen-specific CD4+ T cell response to protein immunization.
pubmed:affiliation
Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't