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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1995-2-10
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pubmed:abstractText |
1. We investigated the glycine-induced response in ventromedial hypothalamic (VMH) neurons freshly dissociated from 8- to 12-day-old rats using the nystatin and gramicidin perforated patch recording modes. The nystatin-formed pores in the plasma membrane are permeable for both monovalent cations and anions, whereas those formed by gramicidin are permeable only to monovalent cations. Therefore, when the patch-pipette contains 150 mM Cl- and gramicidin, the physiological intracellular Cl- concentration ([Cl-]i) is undisturbed in the cell-attached condition of the pipette. 2. At holding potentials of -40 to -60 mV, glycine induced inward currents and outward currents in the nystatin and gramicidin perforated patch recording modes, respectively. The values of the half-maximum effective concentration (EC50) and the Hill coefficient in the concentration-response relationships of the glycine responses were 2.9 x 10(-5) M, 1.1, and 4.2 x 10(-5) M, 1.4, respectively. These values were quite similar in both recording modes. 3. The reversal potentials of the glycine responses (EGly) were -1.5 mV in the nystatin perforated patch recording and -75.0 to -24.8 mV in the gramicidin perforated patch recording. 4. Strychnine (3 x 10(-8) M) inhibited the glycine-induced outward currents in a competitive manner and the half-inhibition concentration (IC50) of strychnine on the 10(-4) M glycine-induced response was 1.9 x 10(-8) M. 5. The physiological [Cl-]i in the VMH neurons calculated from the EGly obtained by the gramicidin perforated patch mode ranged from 6.0 to 43.8 mM (n = 28).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Gramicidin,
http://linkedlifedata.com/resource/pubmed/chemical/Nystatin
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-3077
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1530-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7529820-Animals,
pubmed-meshheading:7529820-Cells, Cultured,
pubmed-meshheading:7529820-Chloride Channels,
pubmed-meshheading:7529820-Chlorides,
pubmed-meshheading:7529820-Glycine,
pubmed-meshheading:7529820-Gramicidin,
pubmed-meshheading:7529820-Membrane Potentials,
pubmed-meshheading:7529820-Neurons,
pubmed-meshheading:7529820-Nystatin,
pubmed-meshheading:7529820-Patch-Clamp Techniques,
pubmed-meshheading:7529820-Rats,
pubmed-meshheading:7529820-Rats, Wistar,
pubmed-meshheading:7529820-Synaptic Transmission,
pubmed-meshheading:7529820-Ventromedial Hypothalamic Nucleus
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pubmed:year |
1994
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pubmed:articleTitle |
Glycine response in acutely dissociated ventromedial hypothalamic neuron of the rat: new approach with gramicidin perforated patch-clamp technique.
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pubmed:affiliation |
Department of Neurophysiology, Tohoku University School of Medicine, Sendai, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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