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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1994-12-28
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pubmed:abstractText |
Polytuftsin, a 35-40-unit repeat of the naturally occurring tetrapeptide tuftsin (TKPR), was chemically linked to EENVEHDA and DDEHVEEPTVA repeat sequences of ring-infected erythrocyte surface antigen protein (an asexual blood-stage antigen) of Plasmodium falciparum. These synthetic constructs were tested for their humoral and cellular immune responses in five inbred strains of mice with different genetic backgrounds (H-2a, H-2b, H-2d, H-2k and H-2i). Mice immunized with these constructs showed higher antibody titres, secondary immune responses and antigen-induced T-cell proliferation compared with the peptide dimers alone. Sera from mice immunized with both the constructs inhibited merozoite invasion of erythrocytes in vitro by 60-80% at 1:10 antisera dilution. Polytuftsin alone proved to be a very poor immunogen in our studies, since no anti-tuftsin antibodies could be detected in the sera. Therefore, we conclude that the synthetic constructs described here could be useful for the development of subunit malaria vaccines.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Protozoan,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Protozoan,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Malaria Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Merozoite Surface Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tuftsin,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Conjugate,
http://linkedlifedata.com/resource/pubmed/chemical/polytuftsin,
http://linkedlifedata.com/resource/pubmed/chemical/ring-infected erythrocyte surface...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
819-24
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7526572-Amino Acid Sequence,
pubmed-meshheading:7526572-Animals,
pubmed-meshheading:7526572-Antibodies, Protozoan,
pubmed-meshheading:7526572-Antigens, Protozoan,
pubmed-meshheading:7526572-Antigens, Surface,
pubmed-meshheading:7526572-Cells, Cultured,
pubmed-meshheading:7526572-Dose-Response Relationship, Immunologic,
pubmed-meshheading:7526572-Drug Carriers,
pubmed-meshheading:7526572-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7526572-Epitopes,
pubmed-meshheading:7526572-Lymphocyte Activation,
pubmed-meshheading:7526572-Malaria Vaccines,
pubmed-meshheading:7526572-Merozoite Surface Protein 1,
pubmed-meshheading:7526572-Mice,
pubmed-meshheading:7526572-Mice, Inbred BALB C,
pubmed-meshheading:7526572-Mice, Inbred Strains,
pubmed-meshheading:7526572-Molecular Sequence Data,
pubmed-meshheading:7526572-Phagocytes,
pubmed-meshheading:7526572-Plasmodium falciparum,
pubmed-meshheading:7526572-Polymers,
pubmed-meshheading:7526572-Protein Precursors,
pubmed-meshheading:7526572-Protozoan Proteins,
pubmed-meshheading:7526572-T-Lymphocytes,
pubmed-meshheading:7526572-Tuftsin,
pubmed-meshheading:7526572-Vaccination,
pubmed-meshheading:7526572-Vaccines, Conjugate
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pubmed:year |
1994
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pubmed:articleTitle |
Construction of a synthetic immunogen: use of the natural immunomodulator polytuftsin in malaria vaccines against RESA antigen of Plasmodium falciparum.
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pubmed:affiliation |
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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