Linked Life Data

7524681

Source:http://linkedlifedata.com/resource/pubmed/id/7524681

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-12-29
pubmed:databankReference
pubmed:abstractText
Cartilage matrix is an interacting multicomponent system of collagen fibrils, fibril-associated small proteoglycans, and large proteoglycans and glycoproteins entrapped within the fibrillar network. In order to better understand the relationships between these different components we have constructed short cDNA clones for detection of mRNAs for two major noncollagenous macromolecules of cartilage matrix, aggrecan and tenascin. We subsequently determined their corresponding mRNA levels by Northern analysis in a panel of total RNAs isolated from several newborn mouse tissues. The expression of aggrecan was strictly restricted to cartilages while tenascin mRNA was present at variable levels in most of the tissues studied. The cDNA clones were also used to identify the cells responsible for aggrecan and tenascin production in newborn mouse tissues by in situ hybridization. With this technique aggrecan mRNA was detected in chondrocytes throughout the developing skeleton in a pattern very similar but not identical to those of type II and IX collagen mRNAs. In the newborn mouse skeleton tenascin and aggrecan mRNAs were expressed essentially in a mutually exclusive manner, tenascin transcripts being present in osteoblasts, periosteal and perichondrial cells, and in cells at articular surfaces. None of these cells expressed the cartilage specific collagen or aggrecan genes. The results further suggest different patterns of gene expression in chondrocytes based on their location in the different cartilages.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
1219
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
613-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7524681-Aggrecans, pubmed-meshheading:7524681-Amino Acid Sequence, pubmed-meshheading:7524681-Animals, pubmed-meshheading:7524681-Animals, Newborn, pubmed-meshheading:7524681-Base Sequence, pubmed-meshheading:7524681-Blotting, Northern, pubmed-meshheading:7524681-Bone Development, pubmed-meshheading:7524681-Cartilage, pubmed-meshheading:7524681-Cell Adhesion Molecules, Neuronal, pubmed-meshheading:7524681-DNA, Complementary, pubmed-meshheading:7524681-DNA Probes, pubmed-meshheading:7524681-Extracellular Matrix Proteins, pubmed-meshheading:7524681-In Situ Hybridization, pubmed-meshheading:7524681-Lectins, C-Type, pubmed-meshheading:7524681-Mice, pubmed-meshheading:7524681-Molecular Sequence Data, pubmed-meshheading:7524681-Proteoglycans, pubmed-meshheading:7524681-RNA, Messenger, pubmed-meshheading:7524681-Sequence Homology, Amino Acid, pubmed-meshheading:7524681-Skull, pubmed-meshheading:7524681-Species Specificity, pubmed-meshheading:7524681-Tenascin, pubmed-meshheading:7524681-Tissue Distribution
pubmed:year
1994
pubmed:articleTitle
Analysis of aggrecan and tenascin gene expression in mouse skeletal tissues by northern and in situ hybridization using species specific cDNA probes.
pubmed:affiliation
University of Turku, Department of Molecular Biology and Medical Biochemistry, Finland.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't