Linked Life Data

7523167

Source:http://linkedlifedata.com/resource/pubmed/id/7523167

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1994-11-10
pubmed:abstractText
Mouse bone marrow (BM) was cultured in the presence of recombinant mouse (rm) interleukin-3 (IL-3), rmIL-4, rmIL-5, rmIL-7, purified mouse (m) IL-9, rmIL-10, recombinant human (rh) macrophage-colony-stimulating factors (M-CSF), rm granulocyte-macrophage colony-stimulating factors (GM-CSF) rm stem cell factor (SCF), rh interferon-alpha (IFN-alpha), rmIFN-gamma, and mNGF to determine which cytokine would give rise to mast cells in murine BM cultures. From a starting population of 1 x 10(7) cells, 1.55 x 10(7) mast cells developed within 14 days in cultures supplemented by rmIL-3. No mast cells were seen at day 14 when any of the other cytokines were present alone, except for rmSCF, which supported the growth of < 0.01% of mast cells observed in IL-3-dependent BM cultures. When rmIL-4, -5, -7, -10, mIL-9, rhM-CSF, rmGM-CSF, rmSCF, rhIFN-alpha, -gamma, or mNGF were added to BM cultures in the presence of rmIL-3, mast cell growth increased 200% with the addition of rmSCF, and 10% when rmIL-4 or IL-9 was added. However, the addition of rhM-CSF, rmGM-CSF, rmIFN-gamma, and mNGF decreased the number of mast cells. Mast cell number, as determined by metachromatic stains, generally approximated the number of Fc epsilon RI+ cells as assessed by FACS analysis. Among the cytokines, only rmIL-4 and rmSCF were able to support the survival of mast cell progenitors in the absence of obvious mast cell proliferation, similarly to rmIL-3. Only rmSCF alone, or in combination with rmIL-3 or -4, supported the growth of mast cells from mouse peripheral blood mononuclear cells (PBMC) where the number of mast cell precursors was about 90 per 10(6) PBMC. With time, mouse BM cells cultured in rmIL-3 became more responsive to rmSCF. Taken together, these data demonstrate that IL-3 is a major early mast cell growth factor, that mast cells become more dependent on SCF with time, and that the effects of IL-3 and SCF are upregulated (IL-4) or downregulated (M-CSF, GM-CSF, IFN-gamma) by both growth factors and proinflammatory cytokines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0301-472X
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1147-55
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7523167-Animals, pubmed-meshheading:7523167-Bone Marrow Cells, pubmed-meshheading:7523167-Cell Division, pubmed-meshheading:7523167-Cell Survival, pubmed-meshheading:7523167-Cells, Cultured, pubmed-meshheading:7523167-Cytokines, pubmed-meshheading:7523167-Drug Interactions, pubmed-meshheading:7523167-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:7523167-Hematopoietic Cell Growth Factors, pubmed-meshheading:7523167-Interferon-gamma, pubmed-meshheading:7523167-Interleukin-3, pubmed-meshheading:7523167-Interleukin-4, pubmed-meshheading:7523167-Leukocytes, Mononuclear, pubmed-meshheading:7523167-Macrophage Colony-Stimulating Factor, pubmed-meshheading:7523167-Mast Cells, pubmed-meshheading:7523167-Mice, pubmed-meshheading:7523167-Mice, Inbred BALB C, pubmed-meshheading:7523167-Nerve Growth Factors, pubmed-meshheading:7523167-Recombinant Proteins, pubmed-meshheading:7523167-Stem Cell Factor, pubmed-meshheading:7523167-Time Factors
pubmed:year
1994
pubmed:articleTitle
Demonstration of differential effects of cytokines on mast cells derived from murine bone marrow and peripheral blood mononuclear cells.
pubmed:affiliation
Allergic Diseases Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
pubmed:publicationType
Journal Article, Comparative Study