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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1994-11-8
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pubmed:abstractText |
An immunomagnetic separation system has been used to collect CD34+ cells in mobilized blood after treatment with a nylon-wool column. Cell purities were increased from 2.6% preseparation to 94.6% postseparation, with a mean yield 45.2% (n = 4). Forty percent of CD34+ cells separated by the immunomagnetic procedure formed colonies in the presence of hematopoietic growth factors in a limiting dilution assay. Eleven percent of these clones proliferated to over 10(5) cells and contained megakaryocytes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1061-6128
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
128-33
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7522896-Antigens, CD,
pubmed-meshheading:7522896-Antigens, CD34,
pubmed-meshheading:7522896-Cell Differentiation,
pubmed-meshheading:7522896-Cell Division,
pubmed-meshheading:7522896-Clone Cells,
pubmed-meshheading:7522896-Humans,
pubmed-meshheading:7522896-Immunomagnetic Separation,
pubmed-meshheading:7522896-Indicator Dilution Techniques,
pubmed-meshheading:7522896-Leukocyte Count,
pubmed-meshheading:7522896-Monocytes,
pubmed-meshheading:7522896-Nylons
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pubmed:year |
1994
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pubmed:articleTitle |
Isolation of human CD34+ peripheral blood stem cells using an immunomagnetic positive selection system: prior monocyte depletion using a nylon-wool column.
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pubmed:affiliation |
Blood Transfusion Center, Tokai University Hospital, Kanagawa, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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