Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1994-9-6
|
| pubmed:abstractText |
The role of vif during the establishment of human immunodeficiency virus type 1 (HIV-1) infection of peripheral blood T lymphocytes and monocyte/macrophages was investigated using vif mutants of three HIV-1 proviral DNAs. Vif was found to be essential for the establishment of productive HIV-1 infection in peripheral blood T lymphocytes after cell-free infection with HXB2 and DFCI-HD, a vpr-positive, vpu-positive, nef-positive derivative of HXB2. A chimeric HIV-1 provirus in which the T-cell line-tropic env sequences in DFCI-HD were replaced with the macrophagetropic env of the ADA strain was constructed for studies on the role of vif during the establishment of HIV-1 infection in primary monocyte/macrophages. These studies showed that vif is also essential for the initiation of productive HIV-1 infection in primary monocyte/macrophage cultures after cell-free virus transmission. The DFCI-HD-ADA virus was shown to replicate in the CD4+ T-cell line Molt 4 clone 8 but not in other T-cell or monocytic cell lines, as previously shown for another macrophagetropic strain YU-2 (1), suggesting that this cell line may be useful for future studies on at least some macrophagetropic strains of HIV-1. The finding that vif is essential for the establishment of productive HIV-1 infection in primary T lymphocytes and monocyte/macrophages suggests that vif may be required for HIV-1 transmission and disease pathogenesis during natural infections and thus may be a good target for prophylactic or therapeutic intervention.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, vif,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Core Protein p24,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Reverse Transcriptase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/vif Gene Products, Human...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0894-9255
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
908-15
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7519673-Cell Line,
pubmed-meshheading:7519673-Cells, Cultured,
pubmed-meshheading:7519673-DNA, Viral,
pubmed-meshheading:7519673-Gene Products, vif,
pubmed-meshheading:7519673-Genome, Viral,
pubmed-meshheading:7519673-HIV Core Protein p24,
pubmed-meshheading:7519673-HIV Reverse Transcriptase,
pubmed-meshheading:7519673-HIV-1,
pubmed-meshheading:7519673-HeLa Cells,
pubmed-meshheading:7519673-Humans,
pubmed-meshheading:7519673-Macrophages,
pubmed-meshheading:7519673-Monocytes,
pubmed-meshheading:7519673-Mutation,
pubmed-meshheading:7519673-Open Reading Frames,
pubmed-meshheading:7519673-Proviruses,
pubmed-meshheading:7519673-RNA-Directed DNA Polymerase,
pubmed-meshheading:7519673-T-Lymphocytes,
pubmed-meshheading:7519673-Transfection,
pubmed-meshheading:7519673-Virus Replication,
pubmed-meshheading:7519673-vif Gene Products, Human Immunodeficiency Virus
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Essential role of vif in establishing productive HIV-1 infection in peripheral blood T lymphocytes and monocyte/macrophages.
|
| pubmed:affiliation |
Division of Human Retrovirology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|