Linked Life Data

7519206

Source:http://linkedlifedata.com/resource/pubmed/id/7519206

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-9-1
pubmed:abstractText
The zeta subunit of the TCR complex targets receptor surface expression, is phosphorylated on tyrosine residues upon T cell activation, and is implicated in signal transduction after TCR ligation. Here we show that, although intrathymic expression of the murine TCR-associated zeta-chain relative to the other chains of the Ag receptor complex remains unchanged during early thymocyte development, there is a doubling of TCR-associated zeta-chain surface expression upon thymocyte maturation. The ratio of tyrosine-phosphorylated relative to nonphosphorylated TCR-associated zeta-chain also changes with thymocyte development. This ratio was quantified after the purification and detergent extraction of receptor complexes from freshly isolated immature or mature thymocytes. Immunoprecipitation of the zeta-chain released from the complex allowed for the isolation of the tyrosine-phosphorylated and nonphosphorylated forms of TCR-associated zeta-chain. Intracellular free zeta-chain was characterized by immunoprecipitation after clearing the cell lysate of intact TCR complexes. Densitometric analysis of immunoblots indicated that surface phosphorylated zeta-chain is more abundant in immature relative to mature T cell populations, whereas the inverse is true of intracellular phosphorylated zeta-chain. Surface phosphorylated zeta-chain also migrated at a higher m.w. than its cytoplasmic counterpart, suggesting that it is more highly modified on some or all of its available tyrosines. These findings demonstrate that the stoichiometry and post-translational modification of the TCR complex are regulated, in vivo, and may determine the functional maturation of T cell signaling, selection, and activation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1563-80
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7519206-Animals, pubmed-meshheading:7519206-Animals, Newborn, pubmed-meshheading:7519206-Antibodies, Monoclonal, pubmed-meshheading:7519206-Antigens, Surface, pubmed-meshheading:7519206-Cell Differentiation, pubmed-meshheading:7519206-Cytoplasm, pubmed-meshheading:7519206-Gene Expression, pubmed-meshheading:7519206-Membrane Proteins, pubmed-meshheading:7519206-Mice, pubmed-meshheading:7519206-Mice, Inbred C3H, pubmed-meshheading:7519206-Mice, Inbred C57BL, pubmed-meshheading:7519206-Phosphotyrosine, pubmed-meshheading:7519206-RNA, Messenger, pubmed-meshheading:7519206-Receptors, Antigen, T-Cell, pubmed-meshheading:7519206-Signal Transduction, pubmed-meshheading:7519206-Structure-Activity Relationship, pubmed-meshheading:7519206-T-Lymphocytes, pubmed-meshheading:7519206-Thymus Gland, pubmed-meshheading:7519206-Tyrosine
pubmed:year
1994
pubmed:articleTitle
Developmental regulation of the TCR zeta-chain. Differential expression and tyrosine phosphorylation of the TCR zeta-chain in resting immature and mature T lymphocytes.
pubmed:affiliation
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't