rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1994-7-29
|
pubmed:abstractText |
1. The high threshold, voltage-activated (HVA) calcium current was recorded from acutely isolated rat neocortical pyramidal neurones using the whole-cell patch technique to examine the effect of agents that block P-type calcium channels and to compare their effects to those of omega-conotoxin GVIA (omega-CgTX) and nifedipine. 2. When applied at a saturating concentration (100 nM) the peptide toxins omega-Aga-IVA and synthetic omega-Aga-IVA blocked 31.5 and 33.0% of the HVA current respectively. 3. A saturating concentration of nifedipine (10 microM) inhibited 48.2% of the omega-Aga-IVA-sensitive current, whereas saturating concentrations of both omega-Aga-IVA (100 nM) and omega-CgTX (10 microM) blocked separate specific components of the HVA current. 4. Partially purified funnel web spider toxin (FTX) at a dilution of 1:1000 blocked 81.4% of the HVA current and occluded the inhibitory effect of omega-Aga-IVA. Synthetic FTX 3.3 arginine polyamine (sFTX) at a concentration of 1 mM blocked 61.2% of the HVA current rapidly and reversibly. The effects of sFTX were partially occluded by pre-application of omega-Aga-IVA. We conclude that neither FTX nor sFTX blocked a specific component of the HVA current in these cells. 5. In view of the specificity of omega-Aga-IVA for P-type calcium channels in other preparations and for a specific component of the HVA current in dissociated neocortical neurones we conclude that about 30% of the HVA current in these neurones flow through P-channels.
|
pubmed:grant |
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1281419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1311418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1321648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1348859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1371309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1375634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1380382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1382335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1432051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1683761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1705677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1709205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1712382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1847065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-2162047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-2451016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-2537980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-2560643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-2580308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-5641636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-591911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-6093100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-7506757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-7683720
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0022-3751
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
475
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
197-205
|
pubmed:dateRevised |
2009-11-18
|
pubmed:meshHeading |
pubmed-meshheading:7517449-3-Pyridinecarboxylic acid...,
pubmed-meshheading:7517449-Animals,
pubmed-meshheading:7517449-Calcium Channel Blockers,
pubmed-meshheading:7517449-Calcium Channels,
pubmed-meshheading:7517449-Cerebral Cortex,
pubmed-meshheading:7517449-Female,
pubmed-meshheading:7517449-Male,
pubmed-meshheading:7517449-Neurons,
pubmed-meshheading:7517449-Nifedipine,
pubmed-meshheading:7517449-Peptides,
pubmed-meshheading:7517449-Rats,
pubmed-meshheading:7517449-Rats, Sprague-Dawley,
pubmed-meshheading:7517449-Spider Venoms,
pubmed-meshheading:7517449-omega-Agatoxin IVA,
pubmed-meshheading:7517449-omega-Conotoxin GVIA
|
pubmed:year |
1994
|
pubmed:articleTitle |
P-type calcium channels in rat neocortical neurones.
|
pubmed:affiliation |
Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle 98195.
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|