Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-7-29
pubmed:abstractText
1. The high threshold, voltage-activated (HVA) calcium current was recorded from acutely isolated rat neocortical pyramidal neurones using the whole-cell patch technique to examine the effect of agents that block P-type calcium channels and to compare their effects to those of omega-conotoxin GVIA (omega-CgTX) and nifedipine. 2. When applied at a saturating concentration (100 nM) the peptide toxins omega-Aga-IVA and synthetic omega-Aga-IVA blocked 31.5 and 33.0% of the HVA current respectively. 3. A saturating concentration of nifedipine (10 microM) inhibited 48.2% of the omega-Aga-IVA-sensitive current, whereas saturating concentrations of both omega-Aga-IVA (100 nM) and omega-CgTX (10 microM) blocked separate specific components of the HVA current. 4. Partially purified funnel web spider toxin (FTX) at a dilution of 1:1000 blocked 81.4% of the HVA current and occluded the inhibitory effect of omega-Aga-IVA. Synthetic FTX 3.3 arginine polyamine (sFTX) at a concentration of 1 mM blocked 61.2% of the HVA current rapidly and reversibly. The effects of sFTX were partially occluded by pre-application of omega-Aga-IVA. We conclude that neither FTX nor sFTX blocked a specific component of the HVA current in these cells. 5. In view of the specificity of omega-Aga-IVA for P-type calcium channels in other preparations and for a specific component of the HVA current in dissociated neocortical neurones we conclude that about 30% of the HVA current in these neurones flow through P-channels.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1281419, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1311418, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1321648, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1348859, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1371309, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1375634, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1380382, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1382335, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1432051, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1683761, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1705677, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1709205, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1712382, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-1847065, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-2162047, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-2451016, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-2537980, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-2560643, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-2580308, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-5641636, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-591911, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-6093100, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-7506757, http://linkedlifedata.com/resource/pubmed/commentcorrection/7517449-7683720
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
475
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
197-205
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
P-type calcium channels in rat neocortical neurones.
pubmed:affiliation
Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle 98195.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't