Linked Life Data

7517340

Source:http://linkedlifedata.com/resource/pubmed/id/7517340

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-8-4
pubmed:abstractText
Hepatocytes commonly have double nuclei and polyploidy. Both increase with age. The relationship of the double nuclei to each other has never been investigated at the ultrastructural level. Using a newly developed extraction and embedding technique we observed that the two nuclei were often linked by connecting DNA strands which span the gap between the two nuclei. The DNA nature of these filaments was determined by digestion with DNase. Artifactual intermolecular disulfide bonds were prevented by the employment of iodoacetamide in the extracting medium. It was further shown that partially fused and/or separated nuclei were observed by phase microscopy of living cultures as well as in fixed liver tissue. The results may indicate that one way in which binucleation occurs is through nuclear separation when the nucleus contains multiples of DNA (polyploid).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1122-9497
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
163-72
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
The connection between the nuclei of binucleated hepatocytes: an ultrastructural study.
pubmed:affiliation
Department of Pathology, Harbor-UCLA Medical Center, Torrance 90509.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.