Linked Life Data

7516248

Source:http://linkedlifedata.com/resource/pubmed/id/7516248

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-7-18
pubmed:abstractText
Recent work has established the importance of serum proteins which interact with endotoxin (lipopolysaccharide, LPS) from Gram-negative bacteria. Thus human monocytes are activated after binding LPS complexed with a serum protein. LPS-binding protein (LBP) is a protein present in both normal and acute phase sera which binds LPS with high affinity. We describe the purification of LBP from human acute phase serum. The purification procedures combine preparative isoelectric focusing (IEF) and either preparative polyacrylamide gel electrophoresis (PAGE) or alternatively an anion-exchange chromatographic step using a Mono Q HR 5/5 column. This allows the isolation of biologically active LBP. LBP was characterized by N-terminal sequence analysis and by measuring the biological activity using flow cytometry (fluorescence-activated cell sorter, FACS) and a luminol enhanced chemiluminescence (LECL) assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1572-6495
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
654
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
25-34
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Improved method for preparation of lipopolysaccharide-binding protein from human serum by electrophoretic and chromatographic separation techniques.
pubmed:affiliation
Institute of Immunology and Transfusion Medicine, Medical Faculty, Ernst-Moritz-Arndt-University, Greifswald, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't