Linked Life Data

7514558

Source:http://linkedlifedata.com/resource/pubmed/id/7514558

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1994-6-22
pubmed:databankReference
pubmed:abstractText
Group I and II hepatitis C virus genotypes were determined by a newly developed serological genotyping assay. This assay detected antibodies against group-specific recombinant proteins in the putative NS4 protein region (amino acid no. 1676-1760) by an enzyme-linked immunosorbent assay. This region of the hepatitis C virus peptide has many group-specific amino acids; fewer than 50% of these amino acids are identical between groups I and II. Genotypes determined by the serological genotyping assay were compared with those determined by a method in which the polymerase chain reaction was used in 91 chronic hepatitis patients. The group-specific polymerase chain reaction was performed within the genome region corresponding to the putative NS5 protein, where the group II hepatitis C virus genome is 57 nucleotides longer than that of group I. Among 91 chronic hepatitis C patients who had positive results in the second-generation hepatitis C virus antibody (core and NS3 region) assay, hepatitis C virus RNA was detected in 80 patients by polymerase chain reaction in the 5' untranslated region and in 78 patients by this group-specific polymerase chain reaction. As a result, in 76 of 91 patients (84%) genotypes determined by the serological genotyping assay showed complete agreement with those determined by the group-specific polymerase chain reaction, and none of the patients revealed a group opposite to that of hepatitis C virus genotype. The detection rate of the serological genotyping assay (89 of 91; 98%) was even higher than that of the polymerase chain reaction assay (78 of 91; 86%).(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0270-9139
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1347-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7514558-Adult, pubmed-meshheading:7514558-Aged, pubmed-meshheading:7514558-Amino Acid Sequence, pubmed-meshheading:7514558-Antigens, Viral, pubmed-meshheading:7514558-Base Sequence, pubmed-meshheading:7514558-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:7514558-Epitopes, pubmed-meshheading:7514558-Female, pubmed-meshheading:7514558-Genotype, pubmed-meshheading:7514558-Hepacivirus, pubmed-meshheading:7514558-Hepatitis Antibodies, pubmed-meshheading:7514558-Hepatitis C, pubmed-meshheading:7514558-Hepatitis C Antibodies, pubmed-meshheading:7514558-Humans, pubmed-meshheading:7514558-Male, pubmed-meshheading:7514558-Middle Aged, pubmed-meshheading:7514558-Molecular Sequence Data, pubmed-meshheading:7514558-Polymerase Chain Reaction, pubmed-meshheading:7514558-Recombinant Proteins, pubmed-meshheading:7514558-Sensitivity and Specificity, pubmed-meshheading:7514558-Viral Nonstructural Proteins
pubmed:year
1994
pubmed:articleTitle
Significance of specific antibody assay for genotyping of hepatitis C virus.
pubmed:affiliation
Liver Unit, Tokyo Metropolitan Komagome Hospital, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't