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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1994-6-16
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pubmed:abstractText |
Human monocytes (M phi) require stimulation with substances such as bacterial endotoxin [LPS (lipopolysaccharide)] to produce angiogenic activity. In this study, we report that stimulation of M phi with LPS (5 micrograms/ml) in the absence of L-arginine greatly reduced their production of angiogenic activity, as assessed in vivo in rat corneas and in vitro by chemotaxis of human umbilical vein endothelial cells (HU-VECs). D-Arginine did not substitute for L-arginine in the production of angiogenic activity. The nitric oxide synthase (NO synthase, EC 1.14-13.39) inhibitors NG-monomethyl-L-arginine (L-NMMA) and NG-nitro-L-arginine methyl ester (L-NAME) both inhibited the production of angiogenic activity by LPS-stimulated M phi in the presence of L-arginine, suggesting the involvement of this enzyme in the pathway that generates angiogenic activity. Neither of these substances directly inhibited the M phi-derived angiogenic activity. LPS-induced production of the cytokines tumor necrosis factor alpha (TNF-alpha) and interleukin 8 (IL-8) was not significantly reduced when M phi were incubated in the absence of L-arginine. Similarly, L-NMMA and L-NAME did not significantly reduce the LPS-induced production of these cytokines by M phi in the presence of L-arginine. These results suggest that the LPS-stimulation-dependent generation of angiogenic activity by M phi requires an L-arginine-dependent NO-synthase effector mechanism that may be independent of the generation of TNF-alpha and IL-8.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1281554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1330900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1373522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1378355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1500677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1502182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1516112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1612108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1697637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1697685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1701493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1708538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1715579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1725690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1728298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1784630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1847633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1852778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-1858040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-2113477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-2121882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-2172983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-2416860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-2440047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-2443857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-2456062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-2462419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-2464438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-2548208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-2584226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-3092113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-3257980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-3416299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-3855438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-4816003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-6100475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-6204395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-6269759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-6378739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-6612342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-7181105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-8214577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-839070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7514298-927505
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Endotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NG-Nitroarginine Methyl Ester,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/omega-N-Methylarginine
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4190-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7514298-Humans,
pubmed-meshheading:7514298-Animals,
pubmed-meshheading:7514298-Cornea,
pubmed-meshheading:7514298-Rats,
pubmed-meshheading:7514298-Arginine,
pubmed-meshheading:7514298-Endotoxins,
pubmed-meshheading:7514298-Lipopolysaccharides,
pubmed-meshheading:7514298-Umbilical Veins,
pubmed-meshheading:7514298-Monocytes,
pubmed-meshheading:7514298-Cells, Cultured,
pubmed-meshheading:7514298-Endothelium, Vascular,
pubmed-meshheading:7514298-Amino Acid Oxidoreductases,
pubmed-meshheading:7514298-Stereoisomerism
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