Linked Life Data

7512079

Source:http://linkedlifedata.com/resource/pubmed/id/7512079

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-5-6
pubmed:abstractText
Several human myeloid leukemia cell lines growing in vitro can be induced to differentiate to more mature monocyte/macrophage-like cells by treatment with protein kinase C-activating phorbol esters, such as PMA. In addition to PMA, cells of the THP-1 myeloid leukemia cell line acquire macrophage-like characteristics after treatment with all-trans retinoic acid (RA). To analyze the signal transduction mechanisms induced by RA, we first compared the effects of PMA and RA on the expression of genes which are known to be regulated during monocytic differentiation. Both RA and PMA effectively down-regulated c-myc expression, while c-myb expression decreased only after PMA treatment. Expression of the beta 2-integrin genes, CD11a and CD11b, was clearly increased after both of these treatments. Their effects on the src-family tyrosine kinase genes were different: hck expression was similarly induced by these agents but lyn expression was stronger and more rapid after RA treatment. RA also enhanced lyn mRNA production rapidly in HL-60, indicating that the activation of lyn gene expression is common in monocytic and granulocytic maturation of myeloid leukemia cells. To examine whether the AP-1 enhancer activity is involved in RA-induced monocytic differentiation, THP-1 cells were transiently transfected with a chloramphenicol acetyl transferase (CAT)-reporter gene containing 5 copies of the AP-1 binding sites. In contrast to PMA, RA did not induce any CAT activity in these cells, thus suggesting that the RA-induced changes in the expression of those genes described above were not dependent on the AP-1 enhancer activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0020-7136
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
57
pubmed:geneSymbol
c-myb, c-myc, lym
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
98-103
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7512079-Antigens, CD18, pubmed-meshheading:7512079-Cell Differentiation, pubmed-meshheading:7512079-Evaluation Studies as Topic, pubmed-meshheading:7512079-Gene Expression, pubmed-meshheading:7512079-Genes, myc, pubmed-meshheading:7512079-Humans, pubmed-meshheading:7512079-Integrins, pubmed-meshheading:7512079-Leukemia, Myeloid, pubmed-meshheading:7512079-Models, Biological, pubmed-meshheading:7512079-Monocytes, pubmed-meshheading:7512079-Phosphorylation, pubmed-meshheading:7512079-Protein-Tyrosine Kinases, pubmed-meshheading:7512079-Proto-Oncogene Proteins, pubmed-meshheading:7512079-Proto-Oncogene Proteins c-jun, pubmed-meshheading:7512079-Proto-Oncogene Proteins c-myb, pubmed-meshheading:7512079-RNA, Messenger, pubmed-meshheading:7512079-Signal Transduction, pubmed-meshheading:7512079-Tetradecanoylphorbol Acetate, pubmed-meshheading:7512079-Transcription, Genetic, pubmed-meshheading:7512079-Tretinoin, pubmed-meshheading:7512079-Tyrosine
pubmed:year
1994
pubmed:articleTitle
Comparison of retinoic acid and phorbol myristate acetate as inducers of monocytic differentiation.
pubmed:affiliation
Department of Bacteriology and Immunology, University of Helsinki, Finland.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't