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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1994-4-14
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pubmed:databankReference |
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pubmed:abstractText |
We report the cloning of a novel tyrosine kinase (TyK)-encoding gene (TYK) from the human hepatoma cell line Hep3B. Using the polymerase chain reaction (PCR) and oligodeoxyribonucleotide primers based on conserved TYK motifs, a 180-bp fragment was cloned and used to obtain full-length cDNA clones of 2.9 kb, with an open reading frame of 505 amino acids (aa). Restricted expression was detected by Northern blotting or reverse-transcribed PCR in a broad range of cell lines. The predicted aa sequence contains characteristic TyK motifs without a transmembrane region, suggesting an intracellular localization. There was 49% aa sequence identity with human FYN product and 47% with human SRC product; however, several structural differences distinguish this clone from other SRC subfamily members. This clone, FYN-related kinase or FRK, is a novel member of the intracellular TYK gene family.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
138
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pubmed:geneSymbol |
TYK
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
247-51
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7510261-Amino Acid Sequence,
pubmed-meshheading:7510261-Base Sequence,
pubmed-meshheading:7510261-Blotting, Northern,
pubmed-meshheading:7510261-Carcinoma, Hepatocellular,
pubmed-meshheading:7510261-Cell Line,
pubmed-meshheading:7510261-Cloning, Molecular,
pubmed-meshheading:7510261-Conserved Sequence,
pubmed-meshheading:7510261-DNA, Complementary,
pubmed-meshheading:7510261-DNA Primers,
pubmed-meshheading:7510261-Genes, src,
pubmed-meshheading:7510261-Humans,
pubmed-meshheading:7510261-Liver Neoplasms,
pubmed-meshheading:7510261-Molecular Sequence Data,
pubmed-meshheading:7510261-Multigene Family,
pubmed-meshheading:7510261-Neoplasm Proteins,
pubmed-meshheading:7510261-Poly A,
pubmed-meshheading:7510261-Polymerase Chain Reaction,
pubmed-meshheading:7510261-Protein-Tyrosine Kinases,
pubmed-meshheading:7510261-RNA,
pubmed-meshheading:7510261-RNA, Messenger,
pubmed-meshheading:7510261-Sequence Homology, Amino Acid,
pubmed-meshheading:7510261-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Cloning of FRK, a novel human intracellular SRC-like tyrosine kinase-encoding gene.
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pubmed:affiliation |
Genentech Inc., South San Francisco, CA 94080.
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pubmed:publicationType |
Journal Article,
Comparative Study
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