Linked Life Data

7507507

Source:http://linkedlifedata.com/resource/pubmed/id/7507507

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-3-3
pubmed:abstractText
Structural analysis of the promoters of several endothelial genes induced at sites of inflammatory or immune responses reveals binding sites for the transcription factor nuclear factor kappa B (NF-kappa B). Endothelial cells express transcripts encoding the p50/p105 and p65 components of NF-kappa B and the rel-related proto-oncogene c-rel; steady state levels of these transcripts are transiently increased by tumor necrosis factor alpha (TNF-alpha). Western blotting revealed that stimulation of endothelial cells with TNF-alpha resulted in nuclear accumulation of the p50 and p65 components of NF-kappa B. Ultraviolet crosslinking and immunoprecipitation demonstrated binding of the p50 and p65 components of NF-kappa B to the E-selectin kappa B site. Endothelial cells express an inhibitor of NF-kappa B activation, I kappa B-alpha (MAD-3). Protein levels of this inhibitor fall rapidly after TNF-alpha stimulation. In parallel, p50 and p65 accumulate in the nucleus and RNA transcript levels for I kappa B-alpha are dramatically upregulated. Recombinant p65 stimulates expression of E-selectin promoter-reporter constructs. I kappa B-alpha inhibits p65 or TNF-alpha-stimulated E-selectin promoter-reporter gene expression in transfected endothelial cells. The NF-kappa B and I kappa B-alpha system may be an inducible regulatory mechanism in endothelial activation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1281211, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1330325, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1340770, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1372169, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1372388, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1375914, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1379595, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1406630, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-14731549, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1493333, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1533967, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1542686, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1690903, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1691438, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1703529, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1710341, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1713680, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1744583, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-181786, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1829648, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-1986868, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-2001591, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-2018779, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-2191300, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-2203532, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-2225078, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-2250017, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-2263603, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-2666912, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-7692229, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-7694295, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-8096091, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-8319912, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-8334993, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-8352966, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-8386742, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-8452515, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-8460169, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-8497124, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-8497253, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-8505309, http://linkedlifedata.com/resource/pubmed/commentcorrection/7507507-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules, http://linkedlifedata.com/resource/pubmed/chemical/Cytokines, http://linkedlifedata.com/resource/pubmed/chemical/DNA, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/E-Selectin, http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins, http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B, http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor RelA, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
179
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
503-12
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
More...