|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1994-1-24
|
|
pubmed:abstractText |
The requirement for co-stimulation in T-cell activation has become firmly established, whilst the precise identity of the molecules involved remains uncertain. Some of the major co-stimulatory molecules include ICAM-1, LFA-3 and B7. We have investigated the abilities of both LFA-3 and B7 to co-stimulate T-cell proliferation under a number of conditions using transfected Chinese hamster ovary cells. Using anti-CD3 antibodies we observed that B7 but not LFA-3 transfectants were capable of co-stimulating proliferation in purified peripheral blood T cells. In addition, both LFA-3 and B7 could induce proliferation in response to phytohaemagglutinin (PHA) and we obtained additive effects using both B7 and LFA-3 together. Using the superantigen staphylococcal enterotoxin B (SEB), we observed that presentation to purified T cells required the presence of class II-positive transfectants and that sensitivity to antigen was increased approximately 100-fold by the co-transfection of either B7 or LFA-3. However, when co-stimulatory molecules were provided by cells separate from those engaging the T-cell receptor (TcR), only B7 was capable of enhancing proliferation. Kinetic studies which investigated the time dependence for co-stimulation revealed that T cells responding to anti-CD3 antibodies required the B7 co-stimulation within the first few hours, for proliferation to be effective. Our data differentiate between the co-stimulatory abilities of B7 and LFA-3 and support the concept of a pivotal role for B7 in T-cell proliferation.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1349172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1355602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1372649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1377404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1378854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1380180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1383383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1650475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1692079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1713678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1716521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1847722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1847724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1848579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1907921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-1972160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-2113314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-2147950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-2162180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-2540528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-2576417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-2653373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-2834436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-2964908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-3029267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-3143077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-6274961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7505258-7678229
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD2,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Function-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Phytohemagglutinins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0019-2805
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
80
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
242-7
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:7505258-Antigens, CD,
pubmed-meshheading:7505258-Antigens, CD2,
pubmed-meshheading:7505258-Antigens, CD28,
pubmed-meshheading:7505258-Antigens, CD3,
pubmed-meshheading:7505258-Antigens, CD80,
pubmed-meshheading:7505258-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:7505258-Cells, Cultured,
pubmed-meshheading:7505258-Humans,
pubmed-meshheading:7505258-Lymphocyte Activation,
pubmed-meshheading:7505258-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:7505258-Phytohemagglutinins,
pubmed-meshheading:7505258-Receptors, Immunologic,
pubmed-meshheading:7505258-T-Lymphocytes,
pubmed-meshheading:7505258-Time Factors,
pubmed-meshheading:7505258-Transfection
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
B7/CD28 but not LFA-3/CD2 interactions can provide 'third-party' co-stimulation for human T-cell activation.
|
|
pubmed:affiliation |
Bath Institute for Rheumatic Diseases, Trim Bridge, U.K.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
