Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
|
pubmed:dateCreated |
1994-1-14
|
pubmed:abstractText |
CD44 expression and the functional capacity for CD44-dependent binding of hyaluronic acid (HA) were analyzed on unstimulated B cells and on B cells stimulated with a variety of polyclonal B cell activators. Whereas essentially all LPS-activated and anti-IgD-dextran-activated B cells and a subpopulation of IL-5-activated B cells expressed increased levels of cell surface CD44 relative to unstimulated B cells, only IL-5-activated CD44hi B cells constitutively bound to FITC-conjugated hyaluronic acid (FITC-HA). Preincubation of LPS or anti-IgD-dextran-activated B cells with the CD44-specific mAb IRAWB14.4 (IRA) induced a high degree of FITC-HA binding in these populations; preincubation of unstimulated B cells with this CD44-specific mAb induced minimal FITC-HA binding. In contrast, preincubation with mAb IRA failed to induce FITC-HA binding by the IL-5-activated CD44lo B cell subset. Neither the amount of constitutive FITC-HA binding nor the level of IRA-inducible FITC-HA binding correlated simply with the overall level of CD44 expressed by the different B cell populations. Biochemical analysis of immunoprecipitated CD44 molecules revealed that relative to CD44 isolated from all other populations examined, CD44 isolated from IL-5-activated B cells was of a lower molecular weight. Treatment with N-Glycanase eliminated this observed difference in molecular weight, indicating that it reflected differences in N-glycosylation of CD44 on activated B cells. Polymerase chain reaction analysis of amplified cDNA showed that each B cell population expressed a common dominant CD44 mRNA. These findings suggest that post-translational modification of CD44 and/or differential association of CD44 with other cellular components plays a critical role in activation-specific ligand binding by CD44.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD44,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Hyaluronic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lymphocyte Homing
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0022-1767
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
151
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
6712-22
|
pubmed:dateRevised |
2003-11-14
|
pubmed:meshHeading |
pubmed-meshheading:7505013-Animals,
pubmed-meshheading:7505013-Antibodies, Anti-Idiotypic,
pubmed-meshheading:7505013-Antibodies, Monoclonal,
pubmed-meshheading:7505013-Antigens, CD44,
pubmed-meshheading:7505013-B-Lymphocytes,
pubmed-meshheading:7505013-Base Sequence,
pubmed-meshheading:7505013-DNA Primers,
pubmed-meshheading:7505013-Female,
pubmed-meshheading:7505013-Gene Expression,
pubmed-meshheading:7505013-Glycosylation,
pubmed-meshheading:7505013-Hyaluronic Acid,
pubmed-meshheading:7505013-Interleukin-5,
pubmed-meshheading:7505013-Lipopolysaccharides,
pubmed-meshheading:7505013-Lymphocyte Activation,
pubmed-meshheading:7505013-Mice,
pubmed-meshheading:7505013-Mice, Inbred DBA,
pubmed-meshheading:7505013-Molecular Sequence Data,
pubmed-meshheading:7505013-Molecular Weight,
pubmed-meshheading:7505013-Polymerase Chain Reaction,
pubmed-meshheading:7505013-Receptors, Lymphocyte Homing
|
pubmed:year |
1993
|
pubmed:articleTitle |
CD44 expression on activated B cells. Differential capacity for CD44-dependent binding to hyaluronic acid.
|
pubmed:affiliation |
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
|
pubmed:publicationType |
Journal Article
|