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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
35
|
| pubmed:dateCreated |
1994-1-13
|
| pubmed:abstractText |
Hs578T human breast cancer cells are from an estrogen receptor-negative breast cell line derived from a highly aggressive mammary tumor. Our previous insulin-like growth factor binding protein-3 (IGFBP-3) binding studies (Oh, Y., Müller, H. L., Lamson, G., and Rosenfeld, R. G. (1993) J. Biol. Chem. 268, 14964-14971) have demonstrated specific binding of IGFBP-3 on the Hs578T cell surface and a significant inhibitory effect of IGFBP-3, itself, on monolayer growth. In this study, we have demonstrated cell surface association proteins that are specific for IGFBP-3 by showing: 1) detection of 20-, 26-, and 50-kDa proteins by affinity cross-linking with 125I-IGFBP-3E. coli and immunoprecipitation of cell monolayers and cell lysates with anti-IGFBP-3 antibodies; 2) dose-dependent competition of 125I-IGFBP-3E. coli by unlabeled IGFBP-3E. coli; 3) inhibition of IGFBP-3 binding to these cell surface proteins by EDTA and by coincubation with native insulin-like growth factor II (IGF-II), but not by coincubation with [Gln6,Ala7,Tyr18,Leu19,Leu27]IGF-II, an IGF-II analog with decreased affinity for IGFBP-3; and 4) partial purification of 20- and 26-kDa species by IGFBP-3.anti-IGFBP-3 antibody immunoaffinity membranes. Characteristics of these specific IGFBP-3 cell surface association proteins are identical to those observed in our previous monolayer binding assay and monolayer growth assay experiments. The specificity of binding and the inhibitory effect of IGFBP-3 binding on Hs578T cell growth suggest that these cell surface proteins are IGFBP-3-specific receptors or receptor subunits mediating the direct inhibitory effect of IGFBP-3 on monolayer growth of Hs578T cells.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
268
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
26045-8
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7504671-Amino Acid Sequence,
pubmed-meshheading:7504671-Binding Sites,
pubmed-meshheading:7504671-Breast Neoplasms,
pubmed-meshheading:7504671-Carrier Proteins,
pubmed-meshheading:7504671-Cell Membrane,
pubmed-meshheading:7504671-Humans,
pubmed-meshheading:7504671-Insulin-Like Growth Factor Binding Proteins,
pubmed-meshheading:7504671-Molecular Sequence Data,
pubmed-meshheading:7504671-Peptide Fragments,
pubmed-meshheading:7504671-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Demonstration of receptors for insulin-like growth factor binding protein-3 on Hs578T human breast cancer cells.
|
| pubmed:affiliation |
Department of Pediatrics, Stanford University School of Medicine, California 94305.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|