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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2-3
|
| pubmed:dateCreated |
1996-1-11
|
| pubmed:abstractText |
This study describes a reproducible method for the routine determination of amino acids in protein hydrolysates using 9-isothiocyanatoacridine as an HPLC precolumn derivatization reagent. Derivatization is carried out by adding the isothiocyanate dissolved in dry acetonitrile to a buffered amino acid solution without the following extraction of the excess reagent. The resulting N-(9-acridinylthiocarbamoyl)amino acids were separated by one single isocratic HPLC analysis with the UV detection at 280 nm.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0165-022X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
30
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
145-52
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7494091-Acridines,
pubmed-meshheading:7494091-Amino Acids,
pubmed-meshheading:7494091-Calibration,
pubmed-meshheading:7494091-Chromatography, High Pressure Liquid,
pubmed-meshheading:7494091-Indicators and Reagents,
pubmed-meshheading:7494091-Sensitivity and Specificity,
pubmed-meshheading:7494091-Spectrophotometry, Ultraviolet
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
High-performance liquid chromatography of amino acids after derivatization with 9-isothiocyanatoacridine.
|
| pubmed:affiliation |
Department of Biochemistry, Faculty of Sciences, P.J. Safárik University, Kosice, Slovak Republic.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|