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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-1-4
|
| pubmed:abstractText |
Eight 7-month-old pigs were inoculated intradermally with 10(3) plaque-forming units of foot-and-mouth disease virus, type O, and killed 24, 48, 72, or 96 h later. Numerous tissues from each animal were collected and examined histopathologically and by in-situ hybridization to determine the presence of virus and its correlation with lesion development. The probe for in-situ hybridization was a biotinylated 500-base negative-sense transcription product corresponding to a portion of the gene encoding polymerase. With this technique, virus was shown to be widely disseminated in all epidermal tissues, regardless of histologically apparent cellular disruption.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0021-9975
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
113
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
51-8
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7490337-Animals,
pubmed-meshheading:7490337-Aphthovirus,
pubmed-meshheading:7490337-Female,
pubmed-meshheading:7490337-Foot-and-Mouth Disease,
pubmed-meshheading:7490337-In Situ Hybridization,
pubmed-meshheading:7490337-Male,
pubmed-meshheading:7490337-Swine,
pubmed-meshheading:7490337-Swine Diseases
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Pathogenesis of foot-and-mouth disease in swine, studied by in-situ hybridization.
|
| pubmed:affiliation |
Foreign Animal Disease Diagnostic Laboratory, NVSL-VS-APHIS-USDA, Greenport, NY 11944, USA.
|
| pubmed:publicationType |
Journal Article
|