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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1995-11-24
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pubmed:databankReference |
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pubmed:abstractText |
It has been shown that the monomethylated cap structure plays important roles in pre-mRNA splicing and nuclear export of RNA. As a candidate for the factor involved in these nuclear events we have previously purified an 80 kDa nuclear cap binding protein (NCBP) from a HeLa cell nuclear extract and isolated its full-length cDNA. In this report, in order to obtain a clue to the cellular functions of NCBP, we attempted to identify a factor(s) that interacts with NCBP. Using the yeast two-hybrid system we isolated three clones from a HeLa cell cDNA library. We designated the proteins encoded by these clones NIPs (NCBP interacting proteins). NIP1 and NIP2 have an RNP consensus-type RNA binding domain, whereas NIP3 contains a unique domain of Arg-Glu or Lys-Glu dipeptide repeats. We also show that NCBP requires NIP1 for binding to the cap structure. Possible roles of NIPs in cap-dependent nuclear processes are discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-1522107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-1618896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-2126155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-2148205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-2208274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-2381323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-2440046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-2606355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-3065823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-3238751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-3644239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-3967293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-6828386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-7509815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-7537634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-7538140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-7937105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-8036511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-8069914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7478990-8425219
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3638-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7478990-Amino Acid Sequence,
pubmed-meshheading:7478990-Cloning, Molecular,
pubmed-meshheading:7478990-Dinucleotide Repeats,
pubmed-meshheading:7478990-HeLa Cells,
pubmed-meshheading:7478990-Humans,
pubmed-meshheading:7478990-Molecular Sequence Data,
pubmed-meshheading:7478990-Nuclear Cap-Binding Protein Complex,
pubmed-meshheading:7478990-Nuclear Proteins,
pubmed-meshheading:7478990-RNA, Messenger,
pubmed-meshheading:7478990-RNA Cap-Binding Proteins,
pubmed-meshheading:7478990-RNA Caps,
pubmed-meshheading:7478990-RNA-Binding Proteins,
pubmed-meshheading:7478990-Ribonucleoproteins,
pubmed-meshheading:7478990-Sequence Analysis, DNA
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pubmed:year |
1995
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pubmed:articleTitle |
Identification of the factors that interact with NCBP, an 80 kDa nuclear cap binding protein.
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pubmed:affiliation |
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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